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Genotyping of Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes by Direct Application of PCR-RFLP on Dried Blood without DNA Extraction
Genotyping Polymorphisms in Alcohol and Aldehyde Dehydrogenase Genes PCR-RFLP Dried Blood DNA Extraction
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2010/9/20
We have developed a simple, labor-saving, inexpensive, and rapid single nucleotide polymorphism (SNP) genotyping method that works directly on whole human blood. This single-tube genotyping method wa...
DNA提取的应用与相关技术分析Application of DNA Extraction and Analysis of Its Influence Factors
DNA提取 血液 新鲜组织 石蜡标本 冰冻组织
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2007/12/30
摘要为了分析影响提取DNA的有关因素,采用标准酚—氯仿抽提法和蛋白酶消化法提取DNA。结果表明,平均每mL全血可提取200 ~ 300μgDNA;新鲜组织及OCT包埋冰冻组织提取DNA,平均每0.2g组织可获得200~300μgDNA;直接将石蜡标本提取物制作模版,PCR扩增良好。从4种组织中均获得较为纯净的DNA;OCT对组织DNA无不良影响。
Abstract:To explore infl...
An efficient protocol for genomic DNA extraction from citrus species
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2007/7/28
期刊信息
篇名
An efficient protocol for genomic DNA extraction from citrus species
语种
英文
撰写或编译
作者
Cheng YJ,Guo WW,Yi HL,Pang XM,Deng XX
第一作者单位
刊物名称
Plant Molecular Biology Reporter
页面
21(2): 177-178 (2003
出...