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Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
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2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Discovery of New Ligand Binding Pathways in Myoglobin by Random Mutagenesis
Animals Whales Myoglobin Ligands Genetic Techniques Mutagenesis Binding Sites Amino Acid Sequence Protein Conformation Structure-Activity Relationship
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2016/5/23
A random library of single amino acid mutants of myoglobin was generated using a highly efficient, single-base-misincorporation random mutagenesis method to discover new ligand-binding pathways in myo...
Photosynthetic Reaction Center Mutagenesis via Chimeric Rescue of a Non-functional Rhodobacter capsulatus puf Operon with Sequences from Rhodobacter sphaeroides
photosynthesis recombination bacteria energy transfer antenna
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2016/5/23
Photosynthetically active chimeric reaction centers which utilize genetic information from both Rhodobacter capsulatus and Rb. sphaeroides puf operons were isolated using a novel method termed chimeri...
Characterization of a fatty acid elongase condensing enzyme by site-directed mutagenesis and biochemical analysis
ELONGASE CONDENSING ENZYME
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2015/5/21
Fatty acid elongation is the extension of de novo synthesized fatty acids through a series of four reactions analogous to those of fatty acid synthase. ELOs catalyze the first reaction in the elongati...
Studies of the complex of ribosomal protein L1 with its binding site in 23S rRNA: Modification -interference, mutagenesis and crosslinking
Ribosomal proteins escherichia coli ribosomes the protein spiral protein
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2014/12/11
Interaction of ribosomal protein L1 and the 23S rRNA plays an important role in both the structure and biological activity of the Escherichia coli ribosome. We have minimized the binding site for prot...
Aerobic Uptake of Cholesterol by Ergosterol Auxotrophic Strains in Candida glabrata & Random and Site-Directed Mutagenesis of ERG25 in Saccharomyces cerevisiae
Ergosterol Candida glabrata ERG25
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2015/5/22
Candida albicans and Candida glabrata are opportunistic human pathogens that are the leading cause of fungal infections, which are increasingly becoming the leading cause of sepsis in immunosuppressed...
北京师范大学分子生物学课件CHAPTER 6 Mutations, Mutagenesis and DNA Repair
北京师范大学 分子生物学 课件 Mutations Mutagenesis DNA Repair
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2009/11/14
北京师范大学分子生物学课件CHAPTER 6 Mutations, Mutagenesis and DNA Repair。
细菌感受态细胞摄取和分泌DNA的相关性研究① I. 含有质粒的枯草芽孢杆菌自然感受态缺陷突变株的诱变和分离 Study of the Interrelations Between DNA Uptake and Secreting of Competent Bacterial Cells I. Mutagenesis and Isolation of Natural Competence-deficient Mutant from Containing Plasmid Bacillus subtilis
枯草芽孢杆菌 自然感受态缺陷突变株 诱变 筛选
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2008/1/7
以携带pUB110质粒的枯草芽孢杆菌BR151菌株为出发菌株，利用亚硝基胍作诱变剂，直接在LB平板上进行诱变。从2949个菌落中筛选出一个转化频率低于出发菌株2～3个数量级的突变株，并对其营养缺陷型、UV的敏感性及Km抗性和质粒进行了检测，确证其转化能力降低为自然感受态缺陷而非营养缺陷型的改变或重组缺陷所致。