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识别序列为非回纹对称结构限制核酸酶的正确切割位点The True Recognizing Sequence of the Restriction Enzyme Whose Recognizing Sequence is Nonpalindromic
非回纹对称结构 限制性内切酶 识别序列
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2007/12/29
限制性内切核酸酶的切割识别序列分为回纹对称和非回纹对称结构两类，由于DNA是互补双链， 所以对于识别序列为回纹对称结构的限制酶，其识别序列在DNA的两条链上是一致的， 可以写为一个， 但对于识别序列为非回纹对称结构的限制酶来说，其识别序列应为两个，而一些工具书、参考书中仅写为一个。本文通过一个酶切实验证明其识别序列为两个。同时希望通过本文，敦促一些工具书、参考书更正其错误。
Optimization of Starting Time and Period of Induction and Inducer Concentration in the Production of the Restriction Enzyme EcoRI from Recombinant Escherichia coli 294
EcoRI restriction enzyme induction enzyme purification
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2010/6/30
Induction parameters including inducer concentration, period of induction and the cell concentration at which inducer is to be added to the fermentation broth were optimized in order to increase the y...