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Rapid Isolation of Bacterial Photosynthetic Reaction Centers with an Engineered Poly-Histidine Tag
photosynthetic reaction center poly-histidine tag isolation method (bacterium)
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2016/5/23
A very rapid method for isolating bacterial photosynthetic reaction centers (RCs) of high purity and yield is described. A poly-histidine tag is engineered at the C-terminus of the RC M-subunit, allow...
Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry
Methanogen Anaerobic Digester 16S rRNA mcrA Methanobacterium formicicum
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2016/5/20
A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, ...
Lactic Acid Bacteria in Total Mixed Ration Silage Containing Soybean Curd Residue: Their Isolation, Identification and Ability to Inhibit Aerobic Deterioration
Soybean Curd Residue–Containing TMR Silage Aerobic Deterioration Lactic Acid Bacteria Synergistic Effect
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2016/5/20
We investigated the effects of the predominant lactic acid bacteria (LAB) on the fermentation characteristics and aerobic stability of total mixed ration (TMR) silage containing soybean curd residue (...
Urinary Catecholamine and Cortisol Responses of Japanese Shorthorn Cows to Social Isolation
Urinary Cortisol Urinary Catecholamines Social Isolation
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2016/5/3
This study was performed to investigate the use of urinary catecholamines to monitor changes in the activity of the sympathetic nervous system and to determine the relationship of urinary cortisol and...
Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Spermatogonial Stem Cell SSC Pluripotency Long Term Culture Pig
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2016/4/29
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for ...
Strong Reproductive Isolation between Closely Related Tropical Sea Urchins (genus Echinometra)’
Reproductive Isolation Closely Related
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2015/12/17
Morphological, mitochondrial DNA, and single-copy nuclear DNA differences show
that the tropical sea urchin Echinometra mathaei is composed of at least four
independent gene pools. Evolutionary di...
GENETIC DIVERGENCE, REPRODUCTIVE ISOLATION, AND MARINE SPECIATION
allopatric speciation dispersal molecular evolutio
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2015/12/17
In marine species, high dispersal is often associated with only mild genetic
differentiation over large spatial scales. Despite this generalization, there are
numerous reasons for the accumulation o...
Genetic Evidence for Regional Isolation of Pocillopora Corals from Moorea
Pocillopora Corals from Moorea Regional Isolation
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2015/7/16
Pocillopora is one of the most abundant and widely distributed coral genera, with a geographic range spanning the entire tropical Pacific and Indian Oceans as well as the Red Sea and Arabian Gulf. Acr...
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
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2016/5/18
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, whi...
Isolation and characterization of multicytochrome gene cluster from Shewanella putrefaciens MR-1 involved in iron and manganese reduction
Biological sciences Multicytochrome gene cluster Manganese reduction Iron reduction Schewanella putrefaciens
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2015/1/28
Dissimilatory Fe(III) and Mn(IV) reduction is an anaerobic respiratory process common to many environments. A large number of bacteria that utilize these metals as terminal electron acceptors have bee...
Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
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2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
Influence of salts on selective coagulation of whey proteins and their application in the isolation of β-lactoglobulin
whey protein concentrate electrodialysis precipitation of caseinomacropeptide separation of proteins salting out
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2014/3/28
Whey proteins are an important constituent of milk, especially whey from cheese manufacture and have many valuable functional properties such as foaming and emulsifying ability or gel formation. Some ...
Isolation and Characterization of Novel Denitrifying Bacterium Geobacillus sp. SG-01 Strain from Wood Chips Composted with Swine Manure
Geobacillus thermodenitrificans Nitrates Nitrites Swine Manure Wood Chips
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2016/5/12
Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollut...
Isolation, Screening and Identification of Swine Gut Microbiota with Ochratoxin A Biodegradation Ability
Swine Intestinal Microbiota Enzyme-linked Immunosorbent Assay
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2016/5/9
The potential for ochratoxin A (OTA) degradation by swine intestinal microbiota was assessed in the current study. Intestinal content that was collected aseptically from swine was spiked with 100 ppb ...
Isolation, Identification and Tyrosinase Inhibitory Activities of the Extractives from Allamanda cathartica
Allamanda c athartica Tyrosinase Glabridin Kaempferol Naringenin Allamandicin
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2013/3/8
Tyrosinase inhibitory activity of the extractives from A. cathartica was examined and their new bioactivity and potent active compounds were identified. Five compounds, glabridin, new lignan, kaempfer...