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Towards the isolation of esterase and amidase catalytic antibodies: Examination of germinal center diversity by cloning and sequence analysis of genes encoding monoclonal antibodies elicited by a transition state analogue
Esterase catalytic antibodies amidase amide ester hydrolysis reaction hybrid cells B cells the TSA induced antibodies genes cells cell lines
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2014/12/11
In this study, a transition state analogue (TSA) approach was employed for the isolation of catalytic antibodies with esterase or amidase activity. The TSA used in this study, phosphonamidate, mimics ...
High yield recombinant penicillin G amidase production and export into the growth medium using Bacillus megaterium
penicillin G gene
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2006/12/5
28 November 2006,During the last years B. megaterium was continuously developed as production host for the secretion of proteins into the growth medium. Here, recombinant production and export of B. m...