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Characterization of yeast U14 snoRNA interactions required for rRNA processing, and development of a novel in vivo rDNA system for dissecting ribosome biogenesis
U14 small nucleoli ribosomal RNA DNA bases yeast elements
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2014/12/11
U14 small nucleolar RNA (snoRNA) is required for processing of 18S ribosomal RNA. It was hypothesized that U14 might base pair with 18S RNA through two highly conserved U14 sequence elements known as ...
Studies of the complex of ribosomal protein L1 with its binding site in 23S rRNA: Modification -interference, mutagenesis and crosslinking
Ribosomal proteins escherichia coli ribosomes the protein spiral protein
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2014/12/11
Interaction of ribosomal protein L1 and the 23S rRNA plays an important role in both the structure and biological activity of the Escherichia coli ribosome. We have minimized the binding site for prot...
Changes in the mutual orientation of tRNA and 23S rRNA at the peptidyl transferase center of the ribosome detected by crosslinking of a photoreactive transition-state analog
Amino acids dynamic interaction ribosomes peptide bonds peptidyl transferase ribosomes
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2014/12/11
Dynamic interactions between the amino acid acceptor end of tRNA and the ribosome underlie the synthesis of successive peptide bonds at the peptidyl transferase center (PTC) of the 50S ribosomal subun...
油菜叶绿体16S rRNA 基因前导顺序的一级结构
油菜 叶绿体DNA 16SrRNA 顺序分析
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2008/2/4
摘要质粒pBN119的3.2kb BamHⅠ片段的PvuⅡ-BglⅡ片段全顺序长为840bp，其中含油菜叶绿体16 S rRNA基因5′端的140bp。通过寻找GTTC顺序，发现在395至468位核苷酸之间是tRNA Val基 因；在73至118位核苷酸之间是一个蛋白读框。和已发表的玉米叶绿体16SrRNA前导顺序进行 比较，同样存在三个相应的大肠杆菌RNA聚合酶的结合位点。和大肠杆菌的启动子及相...
摘要在高等植物中，5S rRNA基因一级结构是高度保守的，二级结构也相当一致。通过比较18种裸子植物5S rRNA基因序列和二级结构变异，发现55%的核苷酸位点是可变的，这种变异有68%发生在干区（双链区），其中一些变异，如双链的互补性核苷酸替代，GU配对等能够维系5S rRNA二级结构的稳定性。环区相对保守，这与5S rRNA三级结构折叠或在转录翻译过程中蛋白质、RNA的结合相关。还首次报道了松...