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甲型肝炎病毒衣壳蛋白融合基因植物表达载体的构建与遗传转化柑桔的研究Construction of the Plant Expression Vector with Hepatitis a Capsid Protein Fusion Gene and Genetic Transformation of Citrus.Sinensis Osbeck
甲型肝炎病毒衣壳蛋白融合基因 柑桔 植物表达载体 遗传转化 食用疫苗
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2008/1/20
摘要
基因工程领域的研究进展使得植物体成为具有重要经济价值的药用蛋白的生产体系。以含甲型肝炎病毒结构基因cDNA的克隆载体pCDNAⅡA16为模板，用甲型肝炎病毒衣壳蛋白融合基因特异引物进行PCR扩增，得到全长2.2kb衣壳蛋白融合基因序列。经测序鉴定后正向克隆于植物表达载体pBI121中，衣壳蛋白融合基因位于pBI121质粒T-DNA左右边界区间内，处于CaMV35S启动子控制之下。经限制性内...
Regeneration and Agrobacterium-mediated Transformation Studies in Tomato ( Lycopersicon esculentum Miller)
Tomato regeneration Agrobacterium transformation Lycopersicon
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2010/3/23
An optimized regeneration protocol and a suitable transformation technique is a necessity in obtaining transgenic plants in a given plant species. This work summarizes a simple culture method to obtai...
Agrobacterium tumefaciens-Mediated Transformation of Sesame (Sesamum indicumL.)
Sesamum indicum Agrobacterium tumefaciens virulence transformation
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2010/3/23
Sesamum indicum L. cv. Özberk was infected with various Agrobacterium tumefaciens strains to screen susceptibility to infection by Agrobacteria. Following infection, tumorigenesis was efficient w...