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Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Spermatogonial Stem Cell SSC Pluripotency Long Term Culture Pig
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2016/4/29
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for ...
The role of nitric oxide in parthenogenetic activation of pig oocytes: A review
oocyte parthenogenesis activation nitric oxide
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2015/6/4
Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies fo...
Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor
oocyte pig parthenogenesis activation calcium ionophore nitric oxide
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2015/6/4
Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC)...
Expression and Secretion of the Insulin-like Growth Factor System Components by Pig Liver Cells
IGF IGFBP Gene Expression Hepatocyte Pig
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2016/4/28
The aim of the present study was to delineate the expression and secretion of insulin-like growth factor (IGF) system components by pig liver cells. Hepatocytes were prepared from 3-wk-old weanling pi...
Augmentation of Thermotolerance in Primary Skin Fibroblasts from a Transgenic Pig Overexpressing the Porcine HSP70.2
Heat Shock Protein 70.2 Transgenic Pig Thermotolerance
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2016/4/18
A high environmental temperature affects the economic performance of pigs. Heat shock protein 70 (HSP70) has been reported to participate importantly in thermotolerance. This study aims to produce tra...
Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes
In vitro Fertilization Pig Oocyte Liquid Boar Sperm
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2016/4/18
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
The Relative Centrifugation Force Permits Visualization of the Germinal Vesicle in Pig Oocytes
Oocyte Germinal Vesicle Pig Relative Centrifugation Force
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2016/4/18
Pig oocytes contain high levels of lipids in the ooplasm, which reduces the visibility of the germinal vesicle (GV) under microscopic examination. Therefore, the purposes of this study were to investi...
Biochemical Compositions of Follicular Fluid and the Effects of Culture Conditions on the In Vitro Development of Pig Oocytes
Protein Lipid Cholesterol Sex Hormones IVMFC
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2016/4/12
The aims of this study were, firstly, to analyze the biochemical compositions of serum and follicular fluid (FF) from prepubertal gilts after PMSG (1,000 IU) treatment. The concentrations of total pro...