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The role of nitric oxide in parthenogenetic activation of pig oocytes: A review
oocyte parthenogenesis activation nitric oxide
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2015/6/4
Parthenogenetic activation of mammalian oocytes with artificial stimuli is commonly applied in various reproductive biotechniques, e.g. cloning using nuclear transfer. For this reason, many studies fo...
Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor
oocyte pig parthenogenesis activation calcium ionophore nitric oxide
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2015/6/4
Porcine oocytes matured in vitro were activated for parthenogenetic development using either calcium ionophore (50μM for 10 min) or nitric oxide donor SNAP (2mM for 23.5 hours). Protein kinase C (PKC)...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
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2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Parthenogenetic Activation of Porcine Oocytes and Isolation of Embryonic Stem Cells-like Derived from Parthenogenetic Blastocysts
Parthenogenetic Activation Oocyte Embryonic Stem Cells Porcine
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2016/4/26
These experiments were carried out to optimize the parameters of electrical activation, methods of parthenogenetic activation and embryo culture in vitro and meanwhile to isolate embryonic stem cells-...
Effect of Oocyte Activation Regimens on Ploidy of Nuclear Transfer Embryos Reconstructed with Fetal Fibroblasts in Rabbit
Activation Nuclear Transfer Development Ploidy Rabbit
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2016/4/25
Considerable attention has been focused on the cloning of mammalian embryos, as a consequence of poor development, in order to enhance the application of genetic engineering. Experiments were conducte...