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Green tissue-specific analysis of a cloned rbcS promoter from Lemna gibba
Lemna gibba rbcS SSU5C promoter tissue characterization
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2014/9/22
Many plant genetic engineering taskss require the spatial expression of genes which in turn depends upon the availability of specific promoters. The present paper analyses the green-tissue characteris...
2种PCR方法扩增大豆rbcS启动子5′侧翼序列比较
反向PCR 热不对称交错PCR 大豆 rbcS启动子
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2011/10/21
比较了扩增已知序列5′侧翼序列的2种PCR方法。方法一是反向PCR(IPCR),以大豆基因组酶切后的DNA片段自身环化产物做模板,用2对特异引物反向扩增大豆rbcS启动子5′侧翼序列。方法二是热不对称交错PCR(TAIL-PCR),以大豆基因组DNA为模板,用3个巢式特异性引物分别和简并引物组合进行连续的PCR循环。IPCR和TAIL-PCR 2种试验方法分别扩增获得438 bp和867 bp特异...