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【发明专利】用于检测I型和II型非洲猪瘟病毒的多重荧光PCR引物探针组、方法和应用
检测I型II型 非洲猪瘟病毒 多重荧光 PCR引物 探针组
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2023/12/20
【发明专利】用于检测I型和II型非洲猪瘟病毒的多重荧光PCR引物探针组、方法和应用。
2020年，中国农业科学院哈尔滨兽医研究所国家非洲猪瘟专业实验室在开展非洲猪瘟流行病学监测及病原学研究中发现，我国部分省区出现了低致死率的非洲猪瘟基因II型自然变异流行株。目前，全球范围内非洲猪瘟病毒已鉴定出至少24种基因型。其中亚洲主要流行基因II型。时下，非洲猪瘟尚无商品化疫苗或有效治疗方法，早期诊断及感染猪群扑杀是防控主要策略。因此，开展流行病学监测及流行株的基因组变异、生物学表型及致病力研...
Effect of Endocrine Disrupters on mRNA Expression of Vitellogenin (VTG) II and Very Low Density Lipoprotein (apoVLDL)II in the Liver of Quail Embryos
endocrine disrupters VTG II apoVLDLII quail RT-PCR
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2009/7/31
The present study was conducted to assess estrogenic activity of nonylphenol (NP) and octylphenol (OP) in quail embryos by determining mRNA levels of liver vitellogenin (VTG) II and very low density l...
Changes in the Expression of Major Histocompatibility Complex Class II mRNA in Response to Inoculation with Salmonella enteritidis in Cultured Hen Ovarian Tissue
Salmonella enteritidis MHC-II mRNA ovary follicle hen
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2009/7/31
Salmonellosis caused by Salmonella enteritidis (S. enteritidis) is a worldwide problem in recent years affecting the poultry production as well as public health. The ovarian immunity may play signific...
Changes in the Localization of MHC Class II Positive Cells in Response to Salmonella enteritidis Invasion in the Ovary of Laying Hens
follicles ovary MHC-II+ cells Salmonella enteritidis
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2009/7/31
Major histocompatibility complex class II (MHC-II) molecules play essential roles in the initiation of immune responses against pathogenic agents. Salmonella enteritidis (S. enteritidis) bacteria may ...
Changes in the mRNA Expression of TGFβ Receptor Types II and III in the Anterior Pituitary during Induced Molting in Hens
chicken anterior pituitary TGFβ receptors mRNA expression induced molting
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2009/7/31
The goal of this study was to determine whether TGFβ receptors mRNA were expressed in the chicken anterior pituitary, and their expressions were changed during induced molting by the regulation of fee...
Expression of MHC Class I and II in Growing Ovarian Follicles of Young and Old Laying Hens,Gallus domesticus
age of birds follicular growth gene expression laying hen MHC
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2009/7/31
The aim of this study was to determine whether the expressions of MHC class I and II in the ovarian follicles change with follicular growth and aging of birds. Theca layer of white follicles (WF), the...
Influences of MHC Class II Haplotypes on Avian Influenza Traits in Thai Indigenous Chicken
avian influenza virus major histocompatibility complex thai indigenous chicken
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2009/7/30
Natural infections with influenza viruses have been reported in a variety of animal species including human, pigs, horses, sea mammals, mustelids and birds. Occasionally devastating pandemics occur in...
华南理工大学II型猪链球菌致病分子机理研究取得新突破
II型猪链球菌病 病分子机理
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2008/6/2
从华南理工大学获悉，该校生物科学与工程学院王小宁等协调、参与的II型猪链球菌致病分子机理的研究又取得突破，论文作为研究亮点入选《自然·中国》（Nature China）。
据介绍，由华南理工大学生物科学与工程学院王小宁等协调、参与的II型猪链球菌致病分子机理的研究论文《SalK/SalR，a two-component signal transduction system, is esse...
鸡传染性支气管炎病毒S1基因和鸡II型干扰素基因在重组鸡痘病毒中的共表达
传染性支气管炎病毒 鸡II型干扰素 重组鸡痘病毒
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2008/3/27
将鸡II型干扰素（ChIFNγ）基因和鸡传染性支气管炎病毒S1基因同时插入到鸡痘病毒转移载体中，构建含有这2个基因的鸡痘病毒转移载体pSY-ChIFNγS1。采用脂质体法将该质粒转染鸡痘病毒感染的鸡胚成纤维细胞（CEF），经过8轮蓝斑筛选，得到纯化的能够同时表达鸡II型干扰素和鸡传染性支气管炎病毒S1基因的重组鸡痘病毒rFPV-ChIFNγS1。rFPV-ChIFNγS1接种28日龄的SPF鸡1周...
约束指数的检验及一些理论考虑II.对选择指数的一些理论考虑
约束指数 检验
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2007/12/7
摘要本文确定了最宜指数的范围，定义了超级选择的区间，并将其与无约束、约束及反向选择包括在最宜指数之内，从而证明了最宜指数是意义最广的选择指数，并使得在指数选择中，可以在一定范围内要求其中若干性状的进展取得特定的方向和大小。文中还简化了最宜指数的计算公式，并讨了各种选择指灵敏的应用问题。
Reduced expression of MAD2, BCL2, and MAP kinase activity in pig oocytes after in vitro aging are associated with defects in sister chromatid segregation during meiosis II and embryo fragmentation aft
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2007/7/28
期刊信息
篇名
Reduced expression of MAD2, BCL2, and MAP kinase activity in pig oocytes after in vitro aging are associated with defects in sister chromatid segregation during meiosis II and embryo fragmenta...
鸡传染性支气管炎病毒S1基因和鸡II型干扰素基因在重组鸡痘病毒中的共表达
传染性支气管炎病毒 鸡II型干扰素 重组鸡痘病毒
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2013/12/11
将鸡II型干扰素（ChIFNγ）基因和鸡传染性支气管炎病毒S1基因同时插入到鸡痘病毒转移载体中，构建含有这2个基因的鸡痘病毒转移载体pSY-ChIFNγS1。采用脂质体法将该质粒转染鸡痘病毒感染的鸡胚成纤维细胞（CEF），经过8轮蓝斑筛选，得到纯化的能够同时表达鸡II型干扰素和鸡传染性支气管炎病毒S1基因的重组鸡痘病毒rFPV-ChIFNγS1。rFPV-ChIFNγS1接种28日龄的SPF鸡1周...
鸡传染性支气管炎病毒S1基因和鸡II型干扰素基因在重组鸡痘病毒中的共表达
传染性支气管炎病毒 鸡II型干扰素 重组鸡痘病毒
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2013/12/11
将鸡II型干扰素（ChIFNγ）基因和鸡传染性支气管炎病毒S1基因同时插入到鸡痘病毒转移载体中，构建含有这2个基因的鸡痘病毒转移载体pSY-ChIFNγS1。采用脂质体法将该质粒转染鸡痘病毒感染的鸡胚成纤维细胞（CEF），经过8轮蓝斑筛选，得到纯化的能够同时表达鸡II型干扰素和鸡传染性支气管炎病毒S1基因的重组鸡痘病毒rFPV-ChIFNγS1。rFPV-ChIFNγS1接种28日龄的SPF鸡1周...
本研究旨在探明鸡恒定链跨膜区特定氨基酸在形成MHCⅡIi复合物聚合中的作用特征。用大引物PCR定点突变法将鸡Ii链跨膜区2个氨基酸Gln47和Thr50分别突变为丙氨酸（Ala），再连接到pEGFPC1载体中，构建含IiGFP融合基因的真核表达载体。同时还分别构建了含pDsRed2N1MHCⅡα、pDsRed2N1MHCⅡβ、pEGFP N1MHCⅡα和pEGFP N1MHC...