搜索结果: 1-7 共查到“家畜遗传学 ghrelin”相关记录7条 . 查询时间(0.115 秒)
llele frequency for c.335 A>C polymorphisms in porcine ghrelin/obestatin prepropeptide gene and association analysis with performance traits in various pig breeds
quantitative trait locus GHRL pigs polymorphism genetic marker
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2015/10/9
The allelic frequency of c.335A>C polymorphisms in the porcine ghrelin/obestatin prepropeptide (GHRL) gene was surveyed among six pig breeds and two subspecies of wild boars. The c.335C was the most f...
Ghrelin对绵羊早期体外胚胎发育的影响
Ghrelin 绵羊 胚胎 卵裂率
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2014/7/22
为了探讨Ghrelin对绵羊早期胚胎发育的影响。利用绵羊体外受精技术制备绵羊早期胚胎，并分别将受精胚胎放置在4组培养液中进行发育培养：①梯度添加300、600、1 000、4 000、10 000 ng•mL-1 Ghrelin的培养液；②梯度添加1、10、200、400 ng•mL-1 Ghrelin受体拮抗剂培养液；③含400 ng•mL-1 Ghrelin...
Identification of Immunoreactive Ghrelin and its mRNA in the Oviduct of Laying Japanese Quail,Coturnix japonica
ghrelin immunocytochemistry Japanese quail oviduct RT-PCR
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2009/7/31
The aim of this study was to determine whether ghrelin is produced in the oviduct of Japanese quail. Japanese quail laying hens and 20-days-old immature ones were used. The matured birds were examined...
广西水牛ghrelincDNA的克隆及序列分析
水牛 克隆 序列分析
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2009/7/1
［目的］克隆广西水牛ghrelin的cDNA并对其进行序列分析。［方法］以广西沼泽型水牛的真胃底组织总RNA为模板，用特异性引物扩增ghrelin的cDNA。扩增产物经凝胶回收纯化后与pMD 18T连接并转化大肠杆菌DH5α，转化产物经PCR和双酶切鉴定后筛选出阳性克隆，阳性克隆经5×1 ml LB液体培养基培养鉴定后测序。［结果］成功获得了广西水牛ghrelin 的cDNA，该片段长3...
广西水牛ghrelin cDNA的克隆及序列分析
水牛 克隆 序列分析
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2009/7/1
克隆广西水牛ghrelin 的cDNA并对其进行序列分析。［方法］以广西沼泽型水牛的真胃底组织总RNA为模板，用特异性引物扩增ghrelin的cDNA。扩增产物经凝胶回收纯化后与pMD 18T连接并转化大肠杆菌DH5α，转化产物经PCR和双酶切鉴定后筛选出阳性克隆，阳性克隆经5×1 ml LB液体培养基培养鉴定后测序。［结果］成功获得了广西水牛ghrelin 的cDNA，该片段长33...
根据GenBank中报道的绵羊ghrelin序列设计一对特异性引物，以蒙古绵羊真胃组织中提取的总RNA为模板，采用RT-PCR技术扩增出蒙古绵羊ghrelin的cDNA，并克隆到pTZ19载体中，经限制性内切酶谱和DNA序列测定，证实所克隆的蒙古绵羊ghrelin的cDNA为ghrelin的部分序列，因为用NCBI网站上的BLAST功能将测序结果同已发表绵羊ghrelin序列进行对比，205个碱基...