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Cloning, Expression, Characterization, and Computational Approach for Cross-Reactivity Prediction of Manganese Superoxide Dismutase Allergen from Pistachio Nut
cloning cross-reaction Manganese Superoxide Dismutase (MnSOD) pistachio (Pistacia vera)
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2010/11/12
The MnSOD gene from pistachio was successfully cloned and expressed in E. coli. The purified pistachio MnSOD was recognized by IgE in 10 (40%) out of the 25 sera tested. Our results also showed that t...
ANALYSIS OF EXPRESSION AND AMINO ACID SEQUENCE OF THE ALLERGEN MAG 3 IN TWO SPECIES OF HOUSE DUST MITES – DERMATOPHAGOIDES FARINAE AND D. PTERONYSSINUS (ACARI: ASTIGMATA: PYROGLYPHIDAE)
allergy house dust mites house-dust-mite allergens
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2010/12/22
In the 90s of the XX century, 2 new and important allergens of house dust mites mites were cloned and sequenced: Mag 1 and Mag 3. However, the second allergen has been identifi ed to date only in extr...
Expression of the Recombinant Major Allergen of Salsola kali Pollen (Sal k 1) and Comparison with Its Low-Immunoglobulin E-Binding Mutant
comparison expression mutant Sal k 1 Salsola kali
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2010/11/12
Background: The inhalation of Salsola kali pollen is an important cause of pollinosis during summer and early fall throughout desert and semi-desert areas. Sal k 1 has been previously reported as a ma...
Allergen Immunotherapy in Asthma: Current Status and Future Perspectives
allergic asthma allergic conjunctivitis allergic rhinitis immunotherapy Th2 responses
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2010/11/12
Allergen immunotherapy targets Th2 cells activated by specific allergens, which constitutes the basis of allergic disease. Therefore, this approach has therapeutic potential for a variety of allergic ...
Local Induction of a Specific Th1 Immune Response by Allergen Linked Immunostimulatory DNA in the Nasal Explants of Ragweed-Allergic Subjects
allergen allergic rhinitis CpG motif immunotherapy vaccination
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2010/11/16
RW or Amb-a-1 increased the number of IL-4, IL-5, and IL-13 mRNA+ cells in the tissue compared to medium alone. AIC had similar cytokine mRNA reactivity as control tissue. AIC and TT increased IFNγ-mR...
Cloning and Expression of the Allergen Cro s 2 Profilin from Saffron (Crocus sativus)
allergy cloning Cro s 2 profilin recombinant allergen saffron
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2010/11/16
Background: Profilin is a panallergen that is recognized by IgE in allergic patients. Allergy to saffron (Crocus sativus) pollen has been described in people exposed to its pollen. Saffron contains a ...
Hev b 6.02 Is the Most Important Allergen in Health Care Workers Sensitized Occupationally by Natural Rubber Latex Gloves
allergen health care workers latex allergy latex gloves skin prick test
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2010/11/16
Background: Natural rubber latex (NRL) allergy is a common occupational disease in health care workers (HCW). However, few reports have compared the major allergen of HCWs to those in gloves that are ...
Recognition of T Cell Epitopes Unique to Cha o 2, the Major Allergen in Japanese Cypress Pollen, in Allergic Patients Cross-Reactive to Japanese Cedar and Japanese Cypress Pollen
Cha o 2 cross-reactivity Cry j 2 pollinosis T cell epitope
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2010/11/16
Background: Pollens from species of the Cupressaceae family are one of the most important causes of respiratory allergies worldwide. Many patients with pollinosis have specific IgE to both allergens f...
Biomarkers for Allergen Immunotherapy in Cedar Pollinosis
basophils CD203c cedar pollinosis IgG4 immunotherapy
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2010/11/16
To initiate, monitor, and complete effective immunotherapy, biomarkers to predict and visualize the immune responses are needed. First, we need to identify the right candidate for immunotherapy. Secon...
Allergen-specific T-cell Response in Patients with Phenytoin Hypersensitivity; Simultaneous Analysis of Proliferation and Cytokine Production by Carboxyfluorescein Succinimidyl Ester (CFSE) Dilution Assay
allergen-specific T cell CFSE dilution assay IFN-γ lymphocyte transformation test phenytoin hypersensitivity
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2010/11/18
Phenytoin-specific proliferation may be detected with greater sensitivity by the CFSE dilution assay than the conventional LTT. The assay revealed that both CD4+ and CD4- T cells proliferated and prod...
Effect of Antihistamine Eye Drops on the Conjunctival Provocation Test with Japanese Cedar Pollen Allergen
allergen provocation test allergic conjunctivitis antihistamine eye drops Japanese cedar pollinosis ketotifen fumarate levocabastine hydrochloride
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2010/11/19
Preadministration of antihistamine eye drops suppressed the symptoms induced by the allergen, which suggests that this is an effective early therapy for Japanese cedar pollinosis, if it is started bef...
RANTES Production from Mononuclear Cells in Response to the Specific Allergen in Asthma Patients
asthma chemokine eosinophil mononuclear cells platelets RANTES
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2010/11/19
Background: Eosinophils are considered to be the major inflammatory cells in asthma. Since regulated on activation, normal T expressed and secreted (RANTES) is a potent chemoattractant for various imp...
Efficacy of Levocabastine Hydrochloride Ophthalmic Suspension in the Conjunctival Allergen Challenge Test in Japanese Subjects with Seasonal Allergic Conjunctivitis
allergen challenge test allergic conjunctivitis conjunctival itching levocabastine hydrochloride redness
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2010/11/18
The mean itching score in the levocabastine-treated group was 0.08 ± 0.06, which was significantly lower than the mean score of 1.98 ± 0.16 in the vehicle group (P < 0.0001). The redness and chemosis...
Role of the LTB4/BLT1 Pathway in Allergen-induced Airway Hyperresponsiveness and Inflammation
airway responsiveness cytokines lipid mediators lung inflammation Tcells
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2010/11/18
LTB4, a proinflammatory lipid mediator generated from arachidonic acid through the action of 5-lipoxygenase, has been known for over two decades and is implicated in a wide variety of inflammatory dis...
Purification and characterization of M-177, a 177 kDa allergen, from the house dust mite Dermatophagoides farinae
chromatography Dermatophagoides farinae high-molecular-weight allergen histamine IgE M-177 Mag 3 purification skin test
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2010/11/26
A high molecular weight allergen, M-177, was recently discovered in the house dust mite, Dermatophagoides farinae (D. farinae). The aims of this study were to develop a conventional purification proce...