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Cytochalasin D inhibits penetration of hamster eggs by guinea pig and human spermatozoa
Cytochalasin D inhibits penetration hamster eggs guinea pig human spermatozoa
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2009/2/23
Fertilization experiments using zona-free hamster eggs and spermatozoa from both guinea pig and human were conducted in the presence of cytochalasin D to evaluate the possible role of actin filame...
Germ cell degeneration in the contralateral testis of the guinea pig with unilateral torsion of the spermatic cord. Quantitative and ultrastructural studies
testis torsion quantitation germ cell guinea pig reproduction
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2009/2/23
This study evaluated the long term effects of unilateral torsion of the spermatic cord on the contralateral testis of guinea pigs, employing both fine structural and quantitative studies. Young, adu...
Effect of gossypol acetate on guinea pig epididymal spermatozoa in vivo and their susceptibility to capacitation in vitro
gossypol sperm motility sperm maturation cytoplasmic droplet capacitation acrosome reaction
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2009/2/20
To determine the effects of gossypol acetate on guinea pig epididymal and vas deferens sperm maturity and in vivo susceptibility to in vitro capacitation and the acrosome reaction, we examined sperm...
A study of phospholipase in albumin and its role in inducing the acrosome reaction of guinea pig spermatozoa in vitro
capacitation acrosome reaction spermatozoa
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2009/2/19
Phospholipase activity associated with guinea pig spermatozoa during capacitation and the acrosome reaction (AR) was determined by measuring the production of [14C] glycerylphosphorylcholine (GPC) f...
The Effect of ATP Depletion upon the Acrosome Reaction in Guinea Pig Sperm
sperm motility ATP depletion acrosome
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2009/2/19
The suspension of guinea pig spermatozoa in substrate-free medium results in the cessation of sperm motility and a 94% reduction in adenosine triphosphate (ATP) levels within minutes. ATP depletion wa...
The distribution of connexin 43 is associated with the germ cell differentiation and with the modulation of the Sertoli cell junctional barrier in continual（guinea pig）and seasonal breeders'（mink）testes
Gap junction Cx43 Sertoli cell blood-testis barrier
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2009/2/13
To test whether the gap junction protein connexin 43 (Cx43) is associated with germ cell differentiation and with the Sertoli cell junctional blood barrier, we recorded the temporal changes in its d...
Mitosis in normal adult guinea pig Leydig cells
Mitosis normal adult guinea pig cells
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2009/2/7
In this study, Leydig cells in mitosis in adult guinea pigs were quantified. Testes of adult control guinea pigs (n = 10) were fixed by whole body perfusion with 2.5% glutaraldehyde in cacodylate bu...
Preparation and short-term culture of enriched populations of guinea pig spermatocytes and spermatids
spermatogenesis spermatocyte spermatid
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2009/2/6
In order to study acrosome biogenesis in vitro, the authors developed techniques for the isolation and culture of enriched populations of guinea pig spermatogenic cells using a modification of the t...
Inhibins in the male Gottingen miniature pig：Leydig cells are the predominant source of inhibin B
ELISA immunohistochemistry in situ hybridization
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2008/12/29
The expression of inhibin subunits in the testes of the Gottingen miniature pig was examined by in situ hybridization and immunohistochemistry. In addition, the major forms were determined by enzyme...
Marked species specificity of guinea pig luteinizing hormone：validation of a bioassay
species specificity guinea pig validation of a bioassay
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2008/12/26
The Guinea pig is a potentially interesting alternative small animal model for the study of the neuroendocrine regulation of reproduction. However, appropriate gonadotropin assays are not widely ava...
First Steps in the Development of a Functional Assay for Human Sperm Using Pig Oocytes
Sperm functionality assay species specificity sperm-oocyte interaction heterologous ICSI
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2008/12/24
The use of mammalian oocytes to assess human sperm functionality could be a helpful tool with potential applications in clinical and research programs. In an attempt to develop the pig model, the...
The Proacrosin Binding Protein, sp32, Is Tyrosine Phosphorylated During Capacitation of Pig Sperm
Fertilization p32 acrosin acrosome reaction
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2008/12/23
Mammalian sperm must undergo capacitation, a preparation period in the female reproductive tract or in vitro, in order to fertilize. We have previously described a Mr 32 000 tyrosine phosphorylated ...