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南京农业大学农学院《PNAS》发表水稻抗病遗传育种课题组“The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice”(图)
水稻抗病 遗传育种 转基因育种
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2022/3/23
2021年7月22日,“作物遗传与种质创新国家重点实验室”的水稻抗病遗传育种课题组在《PNAS》在线发表了题为“The effect of RNA polymerase V on 24-nt siRNA accumulation depends on DNA methylation contexts and histone modifications in rice”的研究论文(Article)。...
Structural basis for RNA replication by the SARS-CoV-2 polymerase
2019-nCoV SARS-CoV-2 COVID-19 remdesivir favipiravir polymerase RdRP virus nsp12 nsp8
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2020/6/11
Nucleotide analog inhibitors, including broad-spectrum remdesivir and favipiravir, have shown promise in in vitro assays and some clinical studies for COVID-19 treatment, this despite an incomplete me...
Screening of BCL-2 associated X protein gene polymorphism associated with scrotal hernia in domesticated swine using polymerase chain reaction-restriction fragment length polymorphism
BAX Gene Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) Screening
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2017/3/9
This study was conducted to screen scrotal hernia in domesticated swine from selected breeders in the Philippines. This defect is associated with a cytosine to thymine mutation in the BCL-2 associated...
Rapid sexing of selected Galliformes by polymerase chain reaction
fowl game fowl polymerase chain reaction
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2015/6/23
Vent sexing of one-day-old chicks in commercial hatcheries has long been common practice
and can be highly accurate. However, there are circumstances when this technique is not applicable such
as ...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
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2015/5/25
Therefore, culture-independent detection methods such as the polymerase chain reaction
are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay
...
Mechanisms of recruitment of the CTD phosphatase Rtr1 to RNA polymerase II
Rtr1 RNA polymerase II transcription
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2015/5/25
The C-terminal domain (CTD) of the RNA polymerase II (RNAPII) subunit Rpb1 must exist in a hypophosphorylated state prior to forming a competent transcription initiation complex. However, during trans...
The role of the CTD phosphatase Rrt1 and post-translational modifications in regulation of RNA polymerase II
CTD phosphatase Rtr1 MuDPIT AP-MS
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2015/5/25
RNA polymerase II (RNAPII) is regulated by multiple modifications to the C-terminal domain (CTD) of the largest subunit, Rpb1. This study has focused on the relationship between hyperphosphorylation o...
Rapid Origin Determination of the Northern Mauxia Shrimp (Acetes chinensis) Based on Allele Specific Polymerase Chain Reaction of Partial Mitochondrial 16S rRNA Gene
Allele-specific Polymerase Chain Reaction Origin Identification
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2016/5/18
Acetes chinensis is an economically important shrimp that belongs to the Sergestidae family; following fermentation, A. chinensis′ economic value, however, is low in China, and much of the catch in Ch...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
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2015/4/30
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell cultu...
Unusual fatal avian polyomavirus infection in nestling cockatiels (Nymphicus hollandicus) detected by nested polymerase chain reaction
budgerigar fledgling disease Melopsittacus undulatus parrots viruses PCR
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2015/3/13
High mortality of nestling cockatiels (Nymphicus hollandicus) was observed in one breeding flock in Slovakia. The nestling mortality affected 50% of all breeding pairs. In general, all the nestlings i...
Polymerase chain reaction assay for the diagnosis of experimentally infected pregnant Sprague-Dawley rats with Brucella abortus biotype 1
Sprague-Dawley rats polymerase chain reaction South Korea
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2015/2/5
In order to diagnose the experimentally infected pregnant Sprague-Dawley (SD) rats with Brucella abortus biotype 1 using polymerase chain reaction (PCR) assay, the SD rats were injected subcutaneously...
Identification of Xiphinema vuittenezi by polymerase chain reaction
nematodes Xiphinema vuittenezi PCR ITS primer
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2015/1/30
So far, the identification of the nematode species Xiphinema vuittenezi relied mainly on time-consuming morphological and morphometrical studies. Therefore, a polymerase chain reaction (PCR) protocol ...
RNA-dependent RNA polymerase of turnip crinkle virus and the effect of coat protein on the replication of subviral RNAs
Biological sciences
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2015/1/28
In this dissertation, I report the functions of the TCV coat protein in TCV viral and subviral RNA replication using in vivo and in vitro systems. In chapter 2, I present my results on construction of...
The stability of T7 RNA polymerase elongation complexes and promoter function analysis by in vitro selection
Forward translocation model polymerase complex stability DNA structure
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2014/12/11
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Functional tests of structural models for abortive cycling in T7 RNA polymerase
Pure sciences Biological sciences Abortive cycling RNA polymerase Transcription
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2014/12/10
Transcription initiates when the enzyme binds to the promoter region and melts open an initial transcribing bubble that extends from position -4 to +4. The initiation phase continues until the enzyme ...