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Cloning and Expression of the Allergen Cro s 2 Profilin from Saffron (Crocus sativus)
allergy cloning Cro s 2 profilin recombinant allergen saffron
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2010/11/16
Background: Profilin is a panallergen that is recognized by IgE in allergic patients. Allergy to saffron (Crocus sativus) pollen has been described in people exposed to its pollen. Saffron contains a ...
猪血小板外廓蛋白(profilin)的分离纯化和鉴定
亲和层析 外廓蛋白 血小板 纯化
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2008/1/5
摘要 本文合成了一种聚脯氨酸亲和层析凝胶,并用这种凝胶纯化了猪血小板外廓蛋白。纯化的外廓蛋白在SDS-聚丙烯酰胺凝胶电泳中呈单一蛋白带,分子量14kD;在体外能显著抑制肌动蛋白聚合。
大豆泛变应原profilin基因的克隆、序列分析和表位预测
大豆 泛变应原 profilin 基因克隆
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2015/9/6
通过生物信息学方法对多种植物性食物和花粉泛变应原proflin基因进行比对,利用其中的高度保守区域设计并合成简并引物,通过RTPCR克隆得到一个新的大豆profilin基因(396bp,pI为5.21,MW为14,1kD),序列同源性分析发现该基因和已知的多种植物性食物、花粉变应原profilin有较高的同源性(>75%),因此认为其系大豆蛋白泛变应原profilin,命名为Gly m 3.02...
Recombinant Zea mays profilin forms multimers with pan-allergenic potential
allergy diagnostic multimer oligomerization plant profilin profilin
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2010/11/25
European studies have shown that approximately 20% of all pollen-allergic patients display IgE reactivity to various plant profilins. Profilins are ubiquitous intracellular proteins, with a role in ce...