搜索结果: 61-75 共查到“知识库 多肽与蛋白质生物化学”相关记录626条 . 查询时间(3.333 秒)
Light-Activated Reassembly of Split Green Fluorescent Protein
Tungsten particle Cobalt-cemented tungsten carbide Adhesion Gap Diamond film
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2016/5/25
Truncated green fluorescent protein (GFP) with the 11th β-strand removed is potentially interesting for bioconjugation, imaging, and the preparation of semisynthetic proteins with novel spectroscopic ...
Synthetic Control of Green Fluorescent Protein
CCFL light source micropropagation
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2016/5/25
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncate...
Deconstructing Green Fluorescent Protein
Green Fluorescent Proteins Spectrometry, Fluorescence Protein Structure,Secondary Protein Folding Peptide Fragments Models,Molecular
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2016/5/25
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding t...
Dynamic Stokes Shift in Green Fluorescent Protein Variants
GFP solvation response ultrafast
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2016/5/25
Solvent reorganization around the excited state of a chromophore leads to an emission shift to longer wavelengths during the excited-state lifetime. This solvation response is absent in wild-type gree...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
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2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Model Membrane Systems and Their Applications
SUPPORTED LIPID-BILAYERS ION MASS-SPECTROMETRY GIANT UNILAMELLAR VESICLES MOLECULAR-DYNAMICS BIOLOGICAL-MEMBRANES PHASE-SEPARATION PROTEINS FUSION RECONSTITUTION SIMULATION
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2016/5/25
The complexity of biological membranes has motivated the development of a wide variety of simpler model systems whose size, geometry, and composition can be tailored with great precision. Approaches h...
Vibrational Stark Effect Probes for Nucleic Acids
ternary complex precipitation europium pyridine-2,6-dicarboxylate fluorescence
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2016/5/24
The vibrational Stark effect (VSE) has proven to be an effective method for the study of electric fields in proteins via the use of infrared probes. To explore the use of VSE in nucleic acids, we inve...
Frictional Drag and Electrical Manipulation of Recombinant Proteins in Polymer-Supported Membranes
Polymers Lipids Recombinant Fusion Proteins Green Fluorescent Proteins Membrane Proteins Electrophoresis Membranes,Artificial Osmosis Histidine Models, Chemical
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2016/5/24
We establish a lipid monolayer supported by a polymer interface that offers advantages over conventional solid-supported membranes for determining the frictional drag at the membrane-protein interface...
Site-Specific Conversion of Cysteine Thiols into Thiocyanate Creates an IR Probe for Electric Fields in Proteins
food packaging perfluorinated dietary intake human exposure
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2016/5/24
The nitrile stretching mode of the thiocyanate moiety is a nearly ideal probe for measuring the local electric field arising from the organized environment of the interior of a protein. Nitriles were ...
E-Cadherin Tethered to Micropatterned Supported Lipid Bilayers as a Model for Cell Adhesion
E-Cadherin Tethered Micropatterned Lipid Bilayers Cell Adhesion
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2016/5/24
Cell-cell adhesion is a dynamic process requiring recruitment, binding, and reorganization of signaling proteins in the plane of the plasma membrane. Here, we describe a new system for investigating h...
Enhancement of the Fluorescence of the Blue Fluorescent Proteins by High Pressure or Low Temperature
Enhancement Fluorescence Blue Fluorescent Proteins High Pressure Low Temperature
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2016/5/24
Green fluorescent proteins bearing the Y66H mutation exhibit strongly blue-shifted fluorescence excitation and emission spectra. However, these blue fluorescent proteins (BFPs) have lower quantum yiel...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
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2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Vibrational Stark Effects Calibrate the Sensitivity of Vibrational Probes for Electric Fields in Proteins
transitional cell carcinoma cDNA microarray molecular markers expression profiling tumor classification
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2016/5/24
Infrared spectroscopy is widely used to probe local environments and dynamics in proteins. The introduction of a unique vibration at a specific site of a protein or more complex assembly offers many a...
Polarized Absorption Spectra of Green Fluorescent Protein Single Crystals:Transition Dipole Moment Directions
Animals Scyphozoa Protons Threonine Serine Luminescent Proteins Green Fluorescent Proteins Microscopy, Fluorescence Amino Acid Substitution Spectrometry, Fluorescence
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2016/5/24
Polarized absorption spectra of orthorhombic crystals of wild-type green fluorescent protein (GFP) were measured between 350 and 520 nm to obtain information on the directions of the electronic transi...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
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2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...