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Proteome Analysis of Bovine Longissimus dorsi Muscle Associated with the Marbling Score
Sirloin Skeletal Muscle Marbling Score Proteomics Cattle
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2016/5/10
The breeding value of marbling score in skeletal muscle is an important factor for evaluating beef quality. In the present study, we investigated proteins associated with the breeding value of the mar...
Spatial Heterogeneity of Bacteria: Evidence from Hot Composts by Culture-independent Analysis
Spatial Heterogeneity 16S rRNA Gene Swine Manure Composting)
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2016/5/10
The phylogenetic diversity of the bacteria in hot composting samples collected from three spatial locations was investigated by molecular tools in order to determine the influence of gradient effect o...
Cytokines Expression and Nitric Oxide Production under Induced Infection to Salmonella Typhimurium in Chicken Lines Divergently Selected for Cutaneous Hypersensitivity
Phytohaemagglutinin-P (PHA-P) Nitric Oxide (NO) Peripheral Blood Mononuclear Cells (PBMCs)
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2016/5/10
In the present study, the impact of Salmonella Typhimurium on cell-mediated immunity (CMI) was investigated in 5 week-old immuno divergent broiler lines selected for the high and low response to phyto...
Substantial Effect of Melanin Influencing Factors on In vitro Melanogenesis in Muzzle Melanocytes of Differently Colored Hanwoo
Melanogenesis Nitric Oxide Hanwoo Muzzle Melanocytes Cysteine
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2016/5/10
The present study was designed to investigate the effect of α-melanocyte-stimulating hormone (α-MSH), nitric oxide (NO) and L-cysteine on melanin production and expression of related genes MC1R, Tyr, ...
Differential Expression of Th1- and Th2- Type Cytokines in Peripheral Blood Mononuclear Cells of Murrah Buffalo (Bubalus Bubalis) on TLR2 Induction by B. Subtilis Peptidoglycan
Differential Cytokine Induction Buffalo PBMCs Th1 and Th2 Type Cytokines Bacillus subtilis Peptidoglycan
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2016/5/10
Peripheral blood mononuclear cells (PBMCs) discriminate microbial pathogens and induce T-cell responses of appropriate effector phenotype accordingly. Toll-like receptors (TLRs), in part, mediate this...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
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2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
Sex Determination of Cattle Meat by Polymerase Chain Reaction Amplification of the DEAD Box Protein (DDX3X/DDX3Y) Gene
Cattle Meat Sex Determination Meat Quality DEAD Box Protein Gene
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2016/5/10
Determination of sex origin of cattle meat by fast and reliable molecular methods is an important measure to ensure correct allocation of export refunds particularly in European countries and also fem...
Growth of Ovarian Primary Follicles Retrieved from Neonates of Different Ages and Derivation of Mature Oocytes Following In vitro-Culture
Neonatal Mouse Ovary Primary Follicle In vitro Culture Oocyte Maturation
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2016/5/10
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follic...
Construction of fat1 Gene Expression Vector and Its Catalysis Efficiency in Bovine Fetal Fibroblast Cells
fat1 Gene Expression Catalysis Efficiency Bovine Fetal Fibroblast Cells
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2016/5/9
The FAT-1 protein is an n-3 fatty acid desaturase, which can recognize a range of 18- and 20-carbon n-6 substrates and transform n-6 polyunsaturated fatty acids (PUFAs) into n-3 PUFAs while n-3 PUFAs ...
Molecular Characterization and Expression Pattern of Gene IGFBP-5 in the Cashmere Goat (Capra hircus)
Cashmere Goat IGFBP-5 Expression Pattern Tissue-specific Hair Gene
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2016/5/9
Insulin-like growth factor-binding protein-5 (IGFBP-5) is one of the six members of IGFBP family, important for cell growth, apoptosis and other IGF-stimulated signaling pathways. In order to explore ...
The Signaling Mechanism of TGF-β1 Induced Bovine Mammary Epithelial Cell Apoptosis
TGF-β1 ALK-5 p-Smad2/3 [Ca2+]i Mammary Epithelial Cell
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2016/5/9
The present study showed that Transforming growth factor beta 1 (TGF-β1) can induce apoptosis of bovine mammary epithelial cells. This apoptosis was also observed with phosphorylation of Smad2/3 withi...
双峰驼胎儿耳缘组织成纤维细胞系的建立及其生物学特性鉴定
双峰驼 耳缘组织 成纤维细胞系 生物学特性
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2016/7/20
本研究旨在建立双峰驼胎儿耳缘组织成纤维细胞系。采集双峰驼胎儿耳缘组织,用植块培养法和消化分离法对其进行原代培养,差速消化和差速贴壁法进行继代培养和细胞纯化,并对培养细胞的形态、细胞活力、生长动力学、免疫组织化学、核型、绿色荧光蛋白基因质粒(GFP)转染表达以及微生物污染等生物学特性进行了分析。结果表明,原代和传代细胞形态正常,群体倍增时间(PDT)为47. 2 h,细胞生长较快;不同代数的染色体众...
在纤毛虫无性生殖中， 生命活动受阻时经常会发生形成包囊的现象。研究纤毛虫的包囊现象， 已成为揭示真核细胞的结构与功能、细胞模式形成与控制机理的一个重要方面[1]。目前， 对腹毛目纤毛虫中游仆虫类包囊的形态及其生理生化特征已进行了较系统的研究， 积累了较多的资料[2-11]， 但对其他类纤毛虫的包囊， 例如尾柱虫类和尖毛虫类包囊的研究所见报道不多。本文以尖毛虫类纤毛虫凯氏异毛虫(Allotricha...
建鲤IGFBP 3 基因多态性对生长的影响
建鲤 胰岛素样生长因子结合蛋白3 单核苷酸多态性 增重 相关性
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2012/3/22
胰岛素样生长因子结合蛋白3 (insulin-like growth factor binding protein,IGFBP3)是调节动物生长和代谢的重要基因。本实验查找了建鲤 (Cyprinus carpio var. jian) IGFBP3s基因上的SNP位点。使用PCR-RFLP检测了其中5个位点 (IGFBP3a-I3_G-253T、IGFBP3b-I2_G30T、IGFBP3b-I3...