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最近几年兴起的TALENs和CRISPR/Cas9基因打靶编辑技术是基因工程技术的热点。经打靶编辑后的基因可产生多种类型的突变形式,而大部分为杂合和双等位突变。同源染色体上的纯合突变可用PCR扩增产物直接测序确定,而对于杂合和双等位突变,则需要经过PCR扩增、克隆、和多样品测序等多个繁琐、耗时的步骤才能鉴定出突变基因型。针对这一问题,生命科学学院、亚热带农业生物资源保护与利用国家重点实验室博士生马...
Molecular biology methods offer new opportunities to differentiate, identify and type bacterial species and strains. These methods use the variability of nucleic sequences of genes such as 16S rDNA, b...
2015年1月12日,中国科学院上海生命科学研究院生物化学与细胞生物学研究所胡苹研究员受邀在Cellular and Molecular Life Sciences上在线发表题为“Stem cell activation in skeletal muscle regeneration”的综述论文。该综述论文由胡苹研究员与其博士研究生傅鑫,香港中文大学王华婷教授合作完成,系统总结了在肌肉再生中起决定...
The last decade has witnessed numerous proteomic studies which have predicted and successfully confirmed the existence of extended structurally flexible regions in protein molecules. Parallel to these...
INTRODUCTION AND BACKGROUND Integrating large volumes of data from diverse sources is a formidable challenge for many investigators in the field of molecular biology. Developing efficient methods for ...
Land-based plants—including the fruits and vegetables in your kitchen—produce and respond to hormones in order to survive. Scientists once believed that hormone signaling machinery only existed in the...
Bartonella bacilliformis (Bb) is the bacterial agent of Carrión's disease and is presumed to be transmitted between humans by phlebotomine sand flies, most notably Lutzomyia verrucarum. Carrión's ...
ABSTRACT A MOLECULAR PHYLOGENETIC ASSESSMENT OF PSEUDENDOCLONIUM SEPTEMBER 2007 RICHARD F. MULLINS JR., B.S., UNIVERSITY OF MASSACHUSETTS AMHERST Directed by: Professor Daniel R. Cooley Pseudendocloni...
Near-Infrared Photometric Variability of Stars Toward the Orion A Molecular Cloud     YY Ori  BM Ori       font style='font-size:12px;'> 2014/12/26
We present an analysis of J, H, and Ks time series photometry obtained with the southern 2MASS telescope over a 0.84◦×6◦ region centered near the Trapezium region of the Orion Nebula Clust...
We present the results of the first detailed millimeter and submillimeter molecular line survey of bright-rimmed clouds, observed at FCRAO in the CO (J = 1 → 0), C18O (J = 1 → 0), HCO+ (J = 1 → 0), H1...
We present the results of the first detailed millimeter and submillimeter molecular line survey of bright-rimmed clouds, observed at FCRAO in the CO (J = 1 → 0), C18O (J = 1 → 0), HCO+ (J = 1 → 0), H1...
High-Resolution Imaging of Molecular Line Emission from High-Redshift QSOs     starburst  evolution  radio lines       font style='font-size:12px;'> 2014/12/26
We present moderate- (1'') and high-resolution (02) observations of the CO (2–1) emission at 43 GHz and radio continuum emission at 1.47 GHz from the z = 4.7 QSO BRI 1202-0725 and the z = 4.4 QSO BRI ...
Sensitive limits on the water abundance in cold low-mass molecular cores     formation  submil       font style='font-size:12px;'> 2014/12/26
We present Submillimeter Wave Astronomy Satellite observations of water vapor in two cold starless clouds, B68 and core D in ρ Ophiuchi. Sensitive nondetections of the 110-101 transition of o-H2O are ...
A survey of 557 GHz water vapor emission in the NGC 1333 molecular cloud     NGC 1333  jets and outfl  ows       font style='font-size:12px;'> 2014/12/24
Using NASA's Submillimeter Wave Astronomy Satellite (SWAS), we have examined the production of water in quiescent and shocked molecular gas through a survey of the 556.936 GHz 110-101 transition of or...
High-Density Molecular Gas in the Infrared-bright Galaxy System VV 114     kinematics and dynamics  VV 114       font style='font-size:12px;'> 2014/12/24
The new high-resolution CO (3-2) interferometric map of the IR-bright interacting galaxy system VV 114 observed with the Submillimeter Array reveals a substantial amount of warm and dense gas in the I...

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