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Meridional Circulation Cells and the Source Waters of the Pacific Equatorial Undercurrent
Meridional Circulation Source Waters Pacific Equatorial Undercurrent
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2008/11/6
A 3½-layer model is used to study the meridional circulation cells that provide the source waters of the Pacific Equatorial Undercurrent (EUC). Its three active layers represent tropical, therm...
Studies on the Effect of Maleic Hydrazide on Root Tip Cells and Pollen Fertility in Trigonella foenum-graecum L.
Cytotoxicity Fenugreek Maleic Hydrazide (MH) Mitotic Index (MI)
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2010/3/17
In the present investigation, the effect of maleic hydrazide (MH) has been studied on seed germination, mitosis, and pollen fertility of Trigonella foenum-graecum L. to test its cytotoxicity. Six diff...
Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells
Porcine Embryonic Stem Cells Pluripotent IVF Basic Fibroblast Growth Factor
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2016/4/28
Porcine embryonic stem (ES) cells have a great potential as tools for transgenic animal production and studies of regulation of differentiation genes. Although several studies showed successful deriva...
Inhibition of Human CD8+ Cytotoxic T Lymphocyte (CTL) -mediated Cytotoxicity in Porcine Fetal Fibroblast Cells by Overexpression of Human Cytomegalovirus Glycoprotein Unique Short (US) 2 Gene
Cytotoxic T Lymphocyte (CTL) cells Swine Leukocyte Antigen (SLA)-I Unique Short (US) 2 Xenotransplantation
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2016/4/28
Xenotransplantation of pig organs into humans is a potential solution for the shortage of donor organs for transplantation. However, multiple immune barriers preclude its clinical application. In part...
Expression and Secretion of the Insulin-like Growth Factor System Components by Pig Liver Cells
IGF IGFBP Gene Expression Hepatocyte Pig
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2016/4/28
The aim of the present study was to delineate the expression and secretion of insulin-like growth factor (IGF) system components by pig liver cells. Hepatocytes were prepared from 3-wk-old weanling pi...
Effect of Genistein on Antioxidative Defence System and Membrane Fluidity in Chick Skeletal Muscle Cells
Genistein Lipid Peroxidation Membrane Fluidity Antioxidation Chick Skeletal Muscle Cells
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2016/4/28
This study was conducted to investigate the protective effect of genistein on the antioxidative defence system and membrane fluidity in chick skeletal muscle cells after supplementation with 0, 20, 40...
Comparison of Genetic Parameter Estimates of Total Sperm Cells of Boars between Random Regression and Multiple Trait Animal Models
Genetic Correlation Boar Semen Random Regression Model Multiple Trait Animal Model
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2016/4/28
The objective of this study was to compare random regression model and multiple trait animal model estimates of the (co) variance of total sperm cells over the active lifetime of AI boars. Data were p...
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
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2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
MHC Class II+ (HLA-DP-like) Cells in the Cow Reproductive Tract: I. Immunolocalization and Distribution of MHC Class II+ Cells in Uterus at Different Phases of the Estrous Cycle
MHC Class II Cow Genital Tract Estrous Cycle
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2016/4/27
This study was undertaken to investigate the distribution of major histocompatibility complex class II positive (MHC II+) (HLA-DP-like) cells in the cow uterus (cervix, corpus and cornu uteri) and to ...
European Cells and Materials （ECM）
European Cells and Materials ECM biology biochemistry 生物学 生物化学
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2008/1/16
European Cells & Materials provides an international and interdisciplinary forum for publication and discussion for researchers in the following fields:Interaction of tissues, cells or bacteria with b...
催乳素和外源基因乳腺共表达载体可提高外源基因在哺乳细胞的表达Mammary Gland Dual－Expression Construct Containing Prolactin and Foreign DNAs Can Promote the Expression of Foreign Proteins in Mammary Cells
催乳素 乳铁蛋白 血小板生成素 表达
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2008/1/8
摘要
为探讨应用牛催乳素促进乳腺生物反应器高效表达外源蛋白（如人乳铁蛋白、人血小板生成素）的可行性，构建两种牛催乳素和外源基因乳腺共表达载体。一种为牛催乳素基因和外源基因共用一个6.7 kb山羊β-酪蛋白启动子启动转录，两个基因以内部核糖体进入位点(IRES)连接；另一种为外源基因利用6.7 kb山羊β－酪蛋白启动子，催乳素基因利用2.0 kb山羊β-酪蛋白启动子，分别转录。脂质体转染后，RT...
细菌感受态细胞摄取和分泌DNA的相关性研究① I. 含有质粒的枯草芽孢杆菌自然感受态缺陷突变株的诱变和分离 Study of the Interrelations Between DNA Uptake and Secreting of Competent Bacterial Cells I. Mutagenesis and Isolation of Natural Competence-deficient Mutant from Containing Plasmid Bacillus subtilis
枯草芽孢杆菌 自然感受态缺陷突变株 诱变 筛选
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2008/1/7
以携带pUB110质粒的枯草芽孢杆菌BR151菌株为出发菌株，利用亚硝基胍作诱变剂，直接在LB平板上进行诱变。从2949个菌落中筛选出一个转化频率低于出发菌株2～3个数量级的突变株，并对其营养缺陷型、UV的敏感性及Km抗性和质粒进行了检测，确证其转化能力降低为自然感受态缺陷而非营养缺陷型的改变或重组缺陷所致。
氯化高铁血红素诱导K562细胞中β-珠蛋白基因表达的研究A Study of Inductive Effect of Hemin on Expression of theβ-globin Genes in K562 Cells
氯化高铁血红素 K562细胞 γ-珠蛋白基因 β-珠蛋白基因
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2007/12/29
以绿色荧光蛋白（EGFP）基因为报道基因，构建了在β-珠蛋白启动子驱动及HS2元件调控下的重组表达载体HG，用脂质体转染法将其转染到K562细胞中，并用RT-PCR方法及流式细胞仪检测氯化高铁血红素(Hm)对K562细胞中β-珠蛋白基因表达及重组载体HG在K562细胞中瞬时表达的影响。结果显示：用30μmol/L Hm诱导K562细胞24、48及72h后，不仅其γ-珠蛋白mRNA水平升高，其β-珠...
Influence of cryopreserved olfactory ensheathing cells transplantation on axonal regeneration in spinal cord of adult rats
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2007/7/28
专著信息
书名
Influence of cryopreserved olfactory ensheathing cells transplantation on axonal regeneration in spinal cord of adult rats
语种
英文
撰写或编译
作者
沈慧勇
第一作者单位
出版社
,《Chinese Journal of Traumatology》 7 ( ...
Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation.
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2007/7/28
专著信息
书名
Probing connection of PBS with the photosystems in intact cells of Spirulina platensis by temperature-induced fluorescence fluctuation.
语种
英文
撰写或编译
作者
Donghui Li,Jie Xie,Yuewei Zhao,Jingquan Z...