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Probing Protein Electrostatics with a Synthetic Fluorescent Amino Acid
pteropod aragonite flux East Sea (Sea of Japan)
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2016/5/24
Electrostatics affect virtually all aspects of protein structure and activity and are particularly important in proteins whose primary function is to stabilize charge. Here we introduce a fluorescent ...
Photophysics of DsRed,a Red Fluorescent Protein,from the Ensemble to the Single-Molecule Level
fluorescence emission titanium dioxide zinc oxide ferric oxide solar concentrator
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2016/5/23
DsRed, a tetrameric fluorescent protein cloned from the Discosoma genus of coral, has shown promise as a longer-wavelength substitute for green fluorescent protein (GFP) mutants for in vivo protein la...
Cell adhesion to protein-micropatterned-supported lipid bilayer membranes
micropatterning microcontact printing of proteins supported lipid bilayers cell adhesion endothelial cells
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2016/5/23
A new method for constructing controlled interfaces between cells and synthetic supported lipid bilayer membranes is reported. Microcontact printing is used to define squares and grid lines of fibrone...
Vibrational Stark Spectroscopy of NO Bound to Heme:Effects of Protein Electrostatic Fields on the NO Stretch Frequency
rare earth alloys X-ray diffraction crystal structure phase diagram
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2016/5/23
The vibrational Stark effect measures the effect of an external electric field on the vibrational (IR) spectrum of a molecule. This technique gives quantitative information on the sensitivity of a vib...
The Role of the Distal and Proximal Protein Environments in Controlling the Ferric Spin State and in Stabilizing Thiolate Ligation in Heme Systems:Thiolate Adducts of the Myoglobin H93G Cavity Mutant
salicylic alcohols hydrogen bonds quantum chemistry
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2016/5/23
Recently, heme protein cavity mutants have been engineered in which the proximal coordinating amino acid has been replaced by a smaller, noncoordinating residue leaving a cavity that can be filled by ...
The Electronic Structure of the Chromophore in Green Fluorescent Protein (GFP)
Electronic Structure Chromophore Green Fluorescent Protein GFP
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2016/5/23
The chromophore responsible for the fluorescence from Green Fluorescent Protein (GFP) of the jelly fish Aequorea Victoria1,2 is formed in an autocatalytic, posttranslational cyclization and oxidation ...
Crystal Structure and Photodynamic Behavior of the Blue-emission Variant Y66H/Y145F of Green Fluorescent Protein
Animals Biological Transport Crystallography, X-Ray Green Fluorescent Proteins Hydrogen-Ion Concentration Luminescent Proteins Luminescent Proteins Luminescent Proteins Mutation Peptides Protein Folding Protons Scyphozoa Spectrometry,Fluorescence tructure-Activity Relationship Titrimetry
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2016/5/23
The crystal structure of a blue emission variant (Y66H/Y145F) of the Aequorea victoria green fluorescent protein has been determined by molecular replacement and the model refined. The crystallographi...
On the Origin of Heme Absorption Band Shifts and Associated Protein Structural Relaxation in Myoglobin Following Flash Photolysis
transhydrogenase NAD NADP Escherichia coli proton pump metal ion
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2016/5/23
The role of the protein structural change monitored by absorption band shifts following flash photolysis of CO from myoglobin is discussed in terms of structure-function relationships. Evidence is pre...
Ultra-fast Excited State Dynamics in Green Fluorescent Protein:Multiple States and Proton Transfer
Protons Luminescent Proteins Green Fluorescent Proteins Recombinant Proteins Spectrometry, Fluorescence Temperature Amino Acid Sequence Protein Conformation Structure-Activity Relationship Oxidation-Reduction
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2016/5/23
The green fluorescent protein (GFP) of the jellyfish Aequorea Victoria has attracted widespread interest since the discovery that its chromophore is generated by the autocatalytic, posttranslational c...
Modulation of Protein Function by Exogenous Ligands in Protein Cavities:CO Binding to a Myoglobin Cavity Mutant Containing Unnatural Proximal Ligands
Animals Binding Sites Carbon Monoxide Cloning, Molecular Escherichia coli Heme Kinetics Ligands Magnetic Resonance Spectroscopy Mutagenesis, Site-Directed Myoglobin Myoglobin Point Mutation Protein Conformation ecombinant Proteins Recombinant Proteins Spectroscopy,Fourier Transform Infrared Valine Whales
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2016/5/23
A variety of heterocyclic ligands can be exchanged into the proximal cavity of sperm whale myoglobin mutant H93G, providing a simple method for introduction of the equivalent of unnatural amino acid s...
Dynamics of Protein Relaxation in Site-Specific Mutants of Human Myoglobin
Humans Cysteine Heme Alanine Myoglobin Recombinant Proteins Spectrophotometry Mutagenesis,Site-Directed Amino Acid Sequence Protein Conformation
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2016/5/23
We have recently reported spectroscopic evidence for structural relaxation of myoglobin (Mb) following photodissociation of MbCO [Lambright, D. G., Balasubramanian, S., & Boxer, S. G. (1991) Chem. Phy...
Selective Examination of Heme Protein Azide Ligand-Distal Globin Interactions Vibrational Circular Dichroism
Heme Protein Azide Ligand-Distal Globin Vibrational Circular Dichroism
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2016/5/23
Vibrational circular dichroism (VCD) spectra of the antisymmetric stretch of azide ligated to the heme of a series of evolutionarily diverse and site-direct mutant hemoglobins and myoglobins are anoma...
Dielectric Relaxation in a Protein Matrix
biology and medicine, basic studies chemistry proteins solvation dielectric properties time dependence relaxation fluorescence
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2016/5/23
We report measurements of time-dependent solvation in a protein medium by the fluorescence dynamic Stokes shift method. Picosecond time-correlated single photon counting fluorescence decays on the com...
Protein Relaxation Dynamics in Human Myoglobin
Protein Relaxation Dynamics Human Myoglobin
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2016/5/23
Transient absorption spectra in the Soret region have been measured following the photolysis of human MbCO in 75%(w/w) glycerol:water at 250, 270, and 290 K. The peak of the transient difference spect...
Large Protein-Induced Dipoles for a Symmetric Carotenoid in a Photosynthetic Antenna Complex
Bacterial Proteins Carotenoids Cell Membrane Chemistry, Physical Macromolecular Substances Membrane Potentials Membrane Proteins Photosynthesis Physicochemical Phenomena Rhodobacter sphaeroides Solvents Spectrum Analysis
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2016/5/23
Unusually large electric field effects have been measured for the absorption spectra of carotenoids (spheroidene) in the B800-850 light-harvesting complex from the photosynthetic bacterium Rhodobacter...