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Synthetic Control of Green Fluorescent Protein
CCFL light source micropropagation
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2016/5/25
Semisynthetic green fluorescent proteins (GFPs) can be prepared by producing truncated GFPs recombinantly and assembling them with synthetic beta-strands of GFP. The yield from expressing the truncate...
Deconstructing Green Fluorescent Protein
Green Fluorescent Proteins Spectrometry, Fluorescence Protein Structure,Secondary Protein Folding Peptide Fragments Models,Molecular
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2016/5/25
Green fluorescent protein (GFP) has been reassembled from two pieces, a large fragment 214 amino acids in length that is produced recombinantly (GFP 1-10) and a short synthetic peptide corresponding t...
Dynamic Stokes Shift in Green Fluorescent Protein Variants
GFP solvation response ultrafast
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2016/5/25
Solvent reorganization around the excited state of a chromophore leads to an emission shift to longer wavelengths during the excited-state lifetime. This solvation response is absent in wild-type gree...
Anomalous Negative Fluorescence Anisotropy in Yellow Fluorescent Protein (YFP 10C):Quantitative Analysis of FRET in YFP Dimers
Alzheimer's disease Amyloid beta 40 peptide Human serum albumin Circular dichroism Dissociation constant alpha-Helix
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2016/5/25
Yellow fluorescent protein (YFP) is widely used as a genetically encoded fluorescent marker in biology. In the course of a comprehensive study of this protein, we observed an unusual, negative fluores...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.2.Unusual Photophysical Properties
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e
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2016/5/25
In the preceding accompanying paper [Shu, X., et al. (2007) Biochemistry 46, 12005-12013], the 1.5 A resolution crystal structure of green fluorescent protein (GFP) variant S65T/H148D is presented, an...
Ultrafast Excited-State Dynamics in the Green Fluorescent Protein Variant S65T/H148D.1.Mutagenesis and Structural Studies
Arabidopsis thaliana homogentisate phytyltransferase (HPT/VTE2) Lactuca sativa tocopherol tocopherol cyclase (TC/VTE1) vitamin e.
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2016/5/24
Wild type green fluorescent protein (wt-GFP) and the variant S65T/H148D each exhibit two absorption bands, A and B, which are associated with the protonated and deprotonated chromophores, respectively...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.3.Temperature Dependence of Proton Transfer
Green Fluorescent Proteins Amino Acid Substitution Spectrometry, Fluorescence Kinetics Mutagenesis,Site-Directed Models,Theoretical Genetic Variation Hydrogen-Ion Concentration Thermodynamics Solvents
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2016/5/24
In parts 1 and 2 of this series [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S. Y., Xi, L. X., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Bio...
Polarized Absorption Spectra of Green Fluorescent Protein Single Crystals:Transition Dipole Moment Directions
Animals Scyphozoa Protons Threonine Serine Luminescent Proteins Green Fluorescent Proteins Microscopy, Fluorescence Amino Acid Substitution Spectrometry, Fluorescence
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2016/5/24
Polarized absorption spectra of orthorhombic crystals of wild-type green fluorescent protein (GFP) were measured between 350 and 520 nm to obtain information on the directions of the electronic transi...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.2.Excited-State Dynamics
Animals Protons Deuterium Luminescent Proteins Green Fluorescent Proteins Spectrometry, Fluorescence Biosensing Techniques Kinetics Genetic Variation Hydrogen-Ion Concentration
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2016/5/24
In the preceding paper [Hanson, G. T., McAnaney, T. B., Park, E. S., Rendell, M. E. P., Yarbrough, D. K., Chu, S., Xi, L., Boxer, S. G., Montrose, M. H., and Remington, S. J. (2002) Biochemistry 41, 1...
Green Fluorescent Protein Variants as Ratiometric Dual Emission pH Sensors.1.Structural Characterization and Preliminary Application
Animals Cricetulus Cell Line Threonine Luminescent Proteins Green Fluorescent Proteins Microscopy, Confocal Crystallography, X-Ray Transfection Amino Acid Substitution
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2016/5/24
Novel dual emission, pH-sensitive variants of the green fluorescent protein (GFP) have been constructed and are suitable for ratiometric emission measurements in vivo. This new class of GFPs, termed d...
Probing Protein Electrostatics with a Synthetic Fluorescent Amino Acid
pteropod aragonite flux East Sea (Sea of Japan)
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2016/5/24
Electrostatics affect virtually all aspects of protein structure and activity and are particularly important in proteins whose primary function is to stabilize charge. Here we introduce a fluorescent ...
Photophysics of DsRed,a Red Fluorescent Protein,from the Ensemble to the Single-Molecule Level
fluorescence emission titanium dioxide zinc oxide ferric oxide solar concentrator
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2016/5/23
DsRed, a tetrameric fluorescent protein cloned from the Discosoma genus of coral, has shown promise as a longer-wavelength substitute for green fluorescent protein (GFP) mutants for in vivo protein la...
Cell adhesion to protein-micropatterned-supported lipid bilayer membranes
micropatterning microcontact printing of proteins supported lipid bilayers cell adhesion endothelial cells
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2016/5/23
A new method for constructing controlled interfaces between cells and synthetic supported lipid bilayer membranes is reported. Microcontact printing is used to define squares and grid lines of fibrone...
The Electronic Structure of the Chromophore in Green Fluorescent Protein (GFP)
Electronic Structure Chromophore Green Fluorescent Protein GFP
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2016/5/23
The chromophore responsible for the fluorescence from Green Fluorescent Protein (GFP) of the jelly fish Aequorea Victoria1,2 is formed in an autocatalytic, posttranslational cyclization and oxidation ...
Crystal Structure and Photodynamic Behavior of the Blue-emission Variant Y66H/Y145F of Green Fluorescent Protein
Animals Biological Transport Crystallography, X-Ray Green Fluorescent Proteins Hydrogen-Ion Concentration Luminescent Proteins Luminescent Proteins Luminescent Proteins Mutation Peptides Protein Folding Protons Scyphozoa Spectrometry,Fluorescence tructure-Activity Relationship Titrimetry
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2016/5/23
The crystal structure of a blue emission variant (Y66H/Y145F) of the Aequorea victoria green fluorescent protein has been determined by molecular replacement and the model refined. The crystallographi...