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On the Origin of Heme Absorption Band Shifts and Associated Protein Structural Relaxation in Myoglobin Following Flash Photolysis
transhydrogenase NAD NADP Escherichia coli proton pump metal ion
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2016/5/23
The role of the protein structural change monitored by absorption band shifts following flash photolysis of CO from myoglobin is discussed in terms of structure-function relationships. Evidence is pre...
Ultra-fast Excited State Dynamics in Green Fluorescent Protein:Multiple States and Proton Transfer
Protons Luminescent Proteins Green Fluorescent Proteins Recombinant Proteins Spectrometry, Fluorescence Temperature Amino Acid Sequence Protein Conformation Structure-Activity Relationship Oxidation-Reduction
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2016/5/23
The green fluorescent protein (GFP) of the jellyfish Aequorea Victoria has attracted widespread interest since the discovery that its chromophore is generated by the autocatalytic, posttranslational c...
Modulation of Protein Function by Exogenous Ligands in Protein Cavities:CO Binding to a Myoglobin Cavity Mutant Containing Unnatural Proximal Ligands
Animals Binding Sites Carbon Monoxide Cloning, Molecular Escherichia coli Heme Kinetics Ligands Magnetic Resonance Spectroscopy Mutagenesis, Site-Directed Myoglobin Myoglobin Point Mutation Protein Conformation ecombinant Proteins Recombinant Proteins Spectroscopy,Fourier Transform Infrared Valine Whales
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2016/5/23
A variety of heterocyclic ligands can be exchanged into the proximal cavity of sperm whale myoglobin mutant H93G, providing a simple method for introduction of the equivalent of unnatural amino acid s...
Dynamics of Protein Relaxation in Site-Specific Mutants of Human Myoglobin
Humans Cysteine Heme Alanine Myoglobin Recombinant Proteins Spectrophotometry Mutagenesis,Site-Directed Amino Acid Sequence Protein Conformation
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2016/5/23
We have recently reported spectroscopic evidence for structural relaxation of myoglobin (Mb) following photodissociation of MbCO [Lambright, D. G., Balasubramanian, S., & Boxer, S. G. (1991) Chem. Phy...
Selective Examination of Heme Protein Azide Ligand-Distal Globin Interactions Vibrational Circular Dichroism
Heme Protein Azide Ligand-Distal Globin Vibrational Circular Dichroism
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2016/5/23
Vibrational circular dichroism (VCD) spectra of the antisymmetric stretch of azide ligated to the heme of a series of evolutionarily diverse and site-direct mutant hemoglobins and myoglobins are anoma...
Dielectric Relaxation in a Protein Matrix
biology and medicine, basic studies chemistry proteins solvation dielectric properties time dependence relaxation fluorescence
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2016/5/23
We report measurements of time-dependent solvation in a protein medium by the fluorescence dynamic Stokes shift method. Picosecond time-correlated single photon counting fluorescence decays on the com...
The Effects of Glucagon-like Peptide-2 on the Tight Junction and Barrier Function in IPEC-J2 Cells through Phosphatidylinositol 3-kinase–Protein Kinase B–Mammalian Target of Rapamycin Signaling Pathway
Transepithelial Electrical Resistance Phosphatidylinositol 3-Kinase-Akt-mTOR Signaling Pathway
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2016/5/20
Glucagon-like peptide-2 (GLP-2) is important for intestinal barrier function and regulation of tight junction (TJ) proteins, but the intracellular mechanisms of action remain undefined. The purpose of...
Heat Shock Protein Augmentation of Angelica gigas Nakai Root Hot Water Extract on Adipogenic Differentiation in Murine 3T3-L1 Preadipocytes
Angelica gigas Nakai Heat Shock Heat Shock Protein 3T3-L1 Adipogenesis
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2016/5/19
There is a high association of heat shock on the alteration of energy and lipid metabolism. The alterations associated with thermal stress are composed of gene expression changes and adaptation throug...
Rumen Degradability and Small Intestinal Digestibility of the Amino Acids in Four Protein Supplements
Amino Acid Protein Small Intestinal Digestibility Rumen Degradability Absorbable Amino Acids
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2016/5/19
The supplementation of livestock feed with animal protein is a present cause for public concern, and plant protein shortages have become increasingly prominent in China. This conflict may be resolved ...
Variance Components and Genetic Parameters Estimated for Fat and Protein Content in Individual Months of Lactation: The Case of Tsigai Sheep
Sheep Milk Composition Test Day Model Variance Ratios Heritability Genetic Correlations
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2016/5/19
The objective of this study was to assess variance components and genetic parameters for fat and protein content in Tsigai sheep using multivariate animal models in which fat and protein content in in...
Scientists discover the evolutionary link between protein structure and function
Scientists evolutionary link protein structure function
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2016/5/31
Proteins are more than a dietary requirement. This diverse set of molecules powers nearly all of the cellular operations in a living organism. Scientists may know the structure of a protein or its fun...
Mapping, Tissue Distribution and Polymorphism of Porcine Retinol Binding Protein Genes (RBP5 and RBP7)
Retinol Binding Protein Complete Coding Sequence Porcine Reverse Transcriptase Polymerase Chain Reaction Single Nucleotide Polymorphism Genotype
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2016/4/28
The retinoids (vitamin A and its derivatives) play a critical role in vision, growth, reproduction, cell differentiation and embryonic development. Using the IMpRH panel, porcine cellular retinol bind...
Polymorphism of Insulin-like Growth Factor Binding Protein-4 Gene in 17 Pig Breeds and Its Relationship with Growth Traits
IGFBP-4 Gene Performance Traits Swine PCR-SSCP
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2016/4/26
Insulin-like growth factor binding protein-4 (IGFBP-4) is a member of the IGF super family, and regulates the action of IGFs. The polymorphism of porcine IGFBP-4 gene in 17 pig breeds (total n = 570) ...
Molecular Cloning, mRNA Expression,and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis
Epididymis G-protein Subunit Galphaq [GNAQ] Sheep Testis
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2016/12/28
The reproductive function of G-protein subunit Galphaq (GNAQ), a member of the G protein alpha subunit family, has been extensively studied in humans and rats. However, no data is available on its sta...
How an artificial protein rescues dying cells
artificial protein rescues dying cells
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2016/3/21
A new study from Princeton has revealed how a synthetic protein revives E. coli cells that lack a life-sustaining gene, offering insight into how life can adapt to survive and potentially be...