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In vitro Culture Conditions for the Mouse Preantral Follicles Isolated by Enzyme Treatment
Chromatin Pattern Media Mouse Preantral Follicle Protein
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2016/4/27
In order to investigate the factors affecting the culture of mouse preantral follicles in vitro, we examined the effect of culture media, protein supplements, and culture period on their growth. The o...
Developmental Competence of Intrafollicular Oocytes Derived from Preantral Follicle Culture with Different Protocols after Parthenogenetic Activation
Mouse Secondary Follicle Intrafollicular Oocyte Maturation Parthenogenesis Blastocyst Formation
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2016/4/26
This study was conducted to improve efficiency of a follicle culture system without reducing developmental competence of intrafollicular oocytes. Preantral follicles (100 to 125 m in diameter)...
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
Retrovirus Gene Transfer Electroporation Stem Cell Medium
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2016/4/26
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be re...
Effect of Protein Sources on Rumen Microbial Protein Synthesis Using Rumen Simulated Continuous Culture System
RSCC Urea Casein Soy Protein Microbial Protein Synthesis N Flow
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2016/4/19
A rumen simulated continuous culture (RSCC) system was used to study the influence of supplementation of the three different types of protein sources such as urea, casein and soy protein on rumen micr...
Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes
In vitro Fertilization Pig Oocyte Liquid Boar Sperm
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2016/4/18
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
Influence of Supplemental Enzymes, Yeast Culture and Effective Micro-organism Culture on Gut Micro-flora and Nutrient Digestion at Different Parts of the Rabbit Digestive Tract
Rabbit Digestion Enzymes Probiotics Gut-microflora
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2016/4/15
An experiment of 10 weeks duration was carried out to study the influence of supplemental effective microorganism (EM) culture, yeast culture and enzymes on nutrient digestibility and gut microflora i...
The Investigation of Cell Culture Conditions to Maintain Chicken Embryonic Stem Cells as Totipotent Cells
Embryonic Stem Cells Feeder Layer Cells Chicken ES Cells Chimeras
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2016/4/13
The ES cell can provide a useful system for studying differentiation and development in vitro and a powerful tool for producing transgenic animalds. To investigate the culture condition of chicken emb...
Biochemical Compositions of Follicular Fluid and the Effects of Culture Conditions on the In Vitro Development of Pig Oocytes
Protein Lipid Cholesterol Sex Hormones IVMFC
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2016/4/12
The aims of this study were, firstly, to analyze the biochemical compositions of serum and follicular fluid (FF) from prepubertal gilts after PMSG (1,000 IU) treatment. The concentrations of total pro...
Effect of Mitogens, Supplement of -mercaptoethanol and Fetal Bovine Serum Supplementation in Whole Blood Culture Medium for Bovine Chromosome Analysis
Bovine Chromosome Analysis #NAME? Mitogens Metaphase Plates
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2016/4/8
We investigated the effects of the mitogen supplements of 3 types, pokeweed mitogen (PWM), phytohemagglutinin (PHA) and concanavalin A (ConA), to a whole blood culture system on the number of metaphas...
Secretory Proteins from Goat Oocytes Matured in Culture
Granulosa Cell Monolayer Polyvinyl Alcohol Oocyte Secretory Proteins IVM
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2016/4/8
In this experiment, oocytes were collected from goat ovaries available in slaughterhouse by follicle puncture method. Morphologically culturable type of oocytes which having compact, multilayered cumu...