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Amplification of Porcine SRY Gene for Sex Determination
SRY Gene Sex Determination Sperm Sorting Duplex PCR
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2016/4/29
The separation of X and Y chromosome-bearing sperm is of use in many aspects of livestock maintenance. In this study, we sought to determine the difference in DNA content between X- and Y-bearing sper...
Effect of Partial Substitution of Dietary Spray-dried Porcine Plasma or Fishmeal with Soybean and Shrimp Protein Hydrolysate on Growth Performance, Nutrient Digestibility and Serum Biochemical Parameters of Weanling Piglets
Protein Hydrolysate SDPP Growth Performance Serum Biochemical Parameter Nutrient Digestibility Weaned Piglets
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2016/4/29
The present experiment was conducted to study the effects of partial replacement of spray-dried porcine protein (SDPP) or fish meal with soybean and shrimp protein hydrolysate (SSPH) on growth perform...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
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2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Relationship between Developmental Ability and Cell Number of Day 2 Porcine Embryos Produced by Parthenogenesis or Somatic Cell Nuclear Transfer
Blastomeric Fragmentation Developmental Ability Embryo Quality Parthenogenesis Porcine Somatic Cell Nuclear Transfer
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2016/4/29
In vitro produced porcine embryos have potential application in reproductive biotechnology. However, their development potential has been very low. This study evaluated the in vitro developmental abil...
The Effect of the Addition and Removal of Various Cryoprotectants on the Nuclear Maturation and ATP Content of Immature Porcine Oocytes
Cryoprotectant Porcine Oocytes ATP Content
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2016/4/29
This study was undertaken to investigate the influence of exposure and removal of four different cryoprotectants (CPAs) on the ATP content of cumulus cell-enclosed (COs) and cumulus cell-denuded (DOs)...
Isolation, Expression Pattern, Polymorphism and Association Analysis of Porcine TIAF1 Gene
TIAF1 Porcine Real-time PCR Carcass Traits Meat Quality Traits Deletion Mutation
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2016/4/29
TIAF1 is a TGF-1-induced anti-apoptotic factor that plays a critical role in blocking TNF (tumor necrosis factor) cytotoxicity in mouse fibroblasts and participates in TGF--mediated gr...
Chinese Medicine Granule Affects the Absorption and Transport of Glucose in Porcine Small Intestinal Brush Border Membrane Vesicles under Heat Stress
Chinese Medicine Granule Heat Stress Pig Intestinal BBMVs Glucose Absorption
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2016/4/29
This study was conducted to investigate the effects of Chinese medicine granule (CMG, including Cortex Phellodendron, Atractylodes Rhizome, Agastache Rugosa and Gypsum Fibrosum) on absorption and tran...
Effects of Donor Cell Passage, Size and Type on Development of Porcine Embryos Derived from Somatic Cell Nuclear Transfer
Donor Cell Passage Size Nuclear Transfer Porcine
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2016/4/29
The aim of this study was to investigate the effects of donor cell passage, size and type on the development of nuclear transfer embryos. Porcine cumulus cells, fetal fibroblasts and oviductal epithel...
Efficient Derivation and Long Term Maintenance of Pluripotent Porcine Embryonic Stem-like Cells
Porcine Embryonic Stem Cells Pluripotent IVF Basic Fibroblast Growth Factor
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2016/4/28
Porcine embryonic stem (ES) cells have a great potential as tools for transgenic animal production and studies of regulation of differentiation genes. Although several studies showed successful deriva...
Inhibition of Human CD8+ Cytotoxic T Lymphocyte (CTL) -mediated Cytotoxicity in Porcine Fetal Fibroblast Cells by Overexpression of Human Cytomegalovirus Glycoprotein Unique Short (US) 2 Gene
Cytotoxic T Lymphocyte (CTL) cells Swine Leukocyte Antigen (SLA)-I Unique Short (US) 2 Xenotransplantation
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2016/4/28
Xenotransplantation of pig organs into humans is a potential solution for the shortage of donor organs for transplantation. However, multiple immune barriers preclude its clinical application. In part...
Investigation of Deletion Variation and Methylation Patterns in the 5' LTR of Porcine Endogenous Retroviruses
Pigs Long Terminal Repeat (LTR) Porcine Endogenous Retroviruses (PERVs) Xenotransplantation
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2016/4/28
The xenotransplantation of pig organs and cells can be related with a risk of transmission of infectious diseases to human. Previous findings indicate that the regulatory region of PERV for retroviral...
Tissue Distribution, SNP Detection and Association Study with Immune Traits of Porcine LBP and CD14 Genes
Pig LBP CD14 TLR4 SNP Immune Trait
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2016/4/28
Lipopolysaccharide binding protein (LBP) and CD14 protein play important roles in the defense against infection of Gram-negative bacteria. In the present study, tissue distribution and polymorphism of...
Sequencing, Genomic Structure, Chromosomal Mapping and Association Study of the Porcine ADAMTS1 Gene with Litter Size
Pig Single Nucleotide Polymorphism Radiation Hybrid Mapping Litter Size ADAMTS1
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2016/4/28
A disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif (ADAMTS1) plays a critical role in follicular rupture and represents a major advance in the proteolytic events...
Optimization of Procedure for Efficient Gene Transfer into Porcine Somatic Cells with Lipofection
Lipofection Porcine Developmental Competence Somatic Cell Nuclear Transfer (SCNT)
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2016/4/27
The objective of this study was to establish conditions for transfection of a foreign gene into somatic cells using cationic lipid reagents and to evaluate the effects of transfection on in vitro deve...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
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2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...