搜索结果: 1-15 共查到“理学 Transgenic”相关记录23条 . 查询时间(0.071 秒)
Analysis of integration sites of transgenic sheep generated by lentiviral vectors using next-generation sequencing technology
Gene transfer gene therapy gene transfer the original nucleus
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2015/5/22
The development of new methods to carry out gene transfer has many benefits to several fields, such as gene therapy, agriculture and animal health. The newly established lentiviral vector systems fur...
Transgenic Expression of MsHsp23 Confers Enhanced Tolerance to Abiotic Stresses in Tall Fescue
Tall Fescue Forage Agrobacterium tumefaciens MsHsp23 Transformation
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2016/5/10
Tall fescue (Festuca arundinacea Schreb.) is an important cool season forage plant that is not well suited to extreme heat, salts, or heavy metals. To develop transgenic tall fescue plants with enhanc...
Rapid Identification of Transgenic Cotton (Gossypium hirsutum L.) Plants by Loop-mediated Isothermal Amplification
Bt chitinase CryIA(b) detection loop-mediated isothermal amplification transgenic cotton
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2014/2/25
In an attempt to speed up the process of screening of transgenic cotton (G. hirsutum L.) plants, a visual and rapid loop-mediated isothermal amplification (LAMP) assay was adopted. Genomic DNA was ext...
Production of Transgenic Goats by Sperm-mediated Exogenous DNA Transfer Method
Sperm-mediated Gene Transfer Goat Sperm Cryopreservation Artificial Insemination (AI)
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2016/5/3
In this study, the production of transgenic goats using sperm to integrate exogenous DNA and artificial insemination (AI) was carried out and the technical protocols for sperm-mediated gene transfer (...
Status and Prospect of Transgenic Potato in China
Potato virus X Transgenic Potato
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2009/9/22
As one of the biggest potato producing countries，China has the largest potato growing
areas in the world．In some arid areas or mountainous districts，this crop is regarded as the
staple food，while in...
降低转基因植物外源基因扩散的分子策略 Molecular Strategies for Decreasing the Gene Flow of Transgenic Plants
转基因植物 基因扩散 分子策略
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2008/1/8
摘要
转基因植物可以通过花粉或种子将外源基因转移到其他植物，从而对生态环境造成潜在的危害。如何降低外源基因的扩散已引起了人们的极大关注。目前降低外源基因扩散的方法主要有叶绿体转化、花粉不育、种子不育、闭花受精、无融合生殖、暂时性控制及转基因缓和等。主要对各种方法的原理和优缺点及目前的使用情况进行综述。Abstract: Transgenic plants can transfer foreign...
番茄转录调控因子基因Pti5植物表达载体的构建及其转化烟草的研究Construction of a Plant Expression Vector with Tomato Transcription Factor Gene Pti5 and Studies on Transgenic Tobaccos
Pti5-VP16基因 表达载体 转化 烟草
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2007/12/31
摘要本实验构建了含有CaMV35S启动子控制下的Pti5-VP16基因的植物双元表达载体pBI121UCH1。通过根癌农杆菌叶盘转化法，将Pti5-VP16基因导入烟草SRI中，经卡那霉素筛选，获得了抗性植株。经PCR和Southern印迹分析，表明抗性植株中整合了Pti5-VP16基因，经抗病性鉴定转基因烟草植株的抗病性明显提高。
Abstract:The plasmid pBI121UCH1...
山羊β乳球蛋白基因的克隆及其在转基因小鼠乳汁中的高效表达Goat β-lactoglobulin Gene Cloning and High Expression in the Mammary Gland of Transgenic Mice
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2007/12/31
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白（β-lactoglobulin,BLG）基因，扩增出的两个片段分别克隆到T载体上，利用BLG基因序列自身存在的NarI单酶切位点进行拼接，获得了全长为7.2kb的山羊BLG基因克隆，并构建了它的真核表达载体，经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠，经PCR和Southern印迹分析...
GFP标记在转基因青鳉同系繁殖纯化中的应用Application of GFP Mark on Inbred Depuration of Transgenic Medaka (Oryzias latipes)
绿色荧光蛋白（GFP）基因 显微注射 青鳉 同系繁殖 纯化
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2007/12/31
摘要从载体pBluescript SK+的Nde I位点处插入外源基因片段，构建了含有青鳉（Oryzias latipes）β-肌动蛋白（β-Actin）启动子和绿色荧光蛋白（GFP）标记基因的新表达载体。显微注射实验证实：在青鳉受精卵的单细胞期进行显微注射，标记基因的表达率和胚胎的存活率均较高。对嵌合体的转基因青鳉（F1）进行了两代的杂合体（F2和F3）筛选和一代的纯合体（F4）筛选，获得第4代...
转基因植物中筛选标记基因的利用及消除Using of Selective Marker Gene in Transgenic Plants and Its Removal
转基因植物 筛选标记基因 基因消除
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2007/12/31
摘要在基因转移过程中，人们常常使用标记基因来筛选转化细胞或组织。常用的筛选标记基因尤其是抗生素抗性基因的使用往往对环境及植物体的生长发育产生不良影响，且影响基因多重转化。为了消除这些弊端，一种全新的发展策略即获取无选择标记的转基因植物应运而生。本文主要综述转基因植物中有关筛选标记基因及其消除方法。
Abstract:Selective marker gene is usually used to...
精子介导转基因动物的制备Research on Spermatozoa-mediated Gene Transfer to Product Transgenic Animals
精子 脂质体 转基因动物
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2007/12/30
摘要采用直接注射的方法，将脂质体包裹的含人乳铁蛋白基因的重组质粒pLNCXHLF注入兔睾丸组织和羊输精管中，一个月后分别与正常雌兔及正常的母羊交配。所产仔兔均为活体，经PCR和Southern检测,转基因仔兔阳性率平均为35%(11/31)；羔羊经引物F1/R1系统PCR检测阳性率为33.3％（4/12），F2/R2系统PCR检测阳性率为25%（3/12）。将流产羔羊组织解剖后提取舌、肺、肝脏、肌...
质粒pCAMBIA1301的检测——The Detection of Plasmid pCAMBIA1301 in Transgenic Rice by Arrayed Primer Extension
生物芯片 基因芯片 转基因植物检测 引物延伸芯片法 biochip genechip transgenic plant detection arreyed primer extension
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2007/12/5
摘要用引物延伸芯片法实现对转基因水稻中
生物芯片技术是生物技术和微制造技术的融合， 已广泛用于生命科学的研究及实践、医学科研及临床、药物设计、环境保护、农业、军事等各个领域。而基因芯片是生物芯片中的一种，是指将大量基因探针分子固定于支持物上，然后与标记的样品进行杂交，所以一次可对大量核酸分子进行检测分析，从而解决了传统核酸印迹杂交技术操作复杂、自动化程度低、检测目的分子数量少、效率低等不足。文章...
NaCl Salinity Stress Decreased Bacillus thuringiensis (Bt) Protein Content if Transgenic Bt Cotton (Gossypium hirsutum L.) Seedlings
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2007/7/28
专著信息
书名
NaCl Salinity Stress Decreased Bacillus thuringiensis (Bt) Protein Content if Transgenic Bt Cotton (Gossypium hirsutum L.) Seedlings
语种
英文
撰写或编译
作者
Linjian Jiang,Liusheng Duan,Xiaoli Tian,Baom...
Genetic engineering of the biosynthesis of glycinebetaine enhances photosynthesis against high temperature stress in transgenic tobacco plants
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2007/7/28
期刊信息
篇名
Genetic engineering of the biosynthesis of glycinebetaine enhances photosynthesis against high temperature stress in transgenic tobacco plants
语种
英文
撰写或编译
撰写
作者
Xinghong Yyang,Zheng Liang,Cong...
Simple and effective detection method for gene expression product of transgenic plant
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2007/7/28
期刊信息
篇名
Simple and effective detection method for gene expression product of transgenic plant
语种
英文
撰写或编译
撰写
作者
Xu Jiaxi,Ma Mi,Ma Yuan,Lin Zhongping
第一作者单位
北京大学,中国科学院植物研究所,清华大学
刊物名称
Chinese Science Bu...