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UVA Team finds way to view genes inside living cells,in real time
UVA Team view genes inside living cells real time
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2017/4/27
For Mazhar Adli, the little glowing dots dancing about on the computer screen are nothing less than the fulfillment of a dream. Those fluorescent dots, moving in real time, are set to illuminate our u...
扇贝多肽经由aSMase-JNK通路抑制UVA诱导HaCaT细胞凋亡
扇贝多肽 酸性鞘磷脂酶 JNK 紫外线A 凋亡 HaCaT细胞
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2009/11/13
建立紫外线A(UVA)辐射损伤HaCaT细胞的病理模型, 从酸性鞘磷脂酶-JNK信号通路的角度研究扇贝多肽(Polypeptide from Chlamys farreri, PCF)抑制UVA诱导HaCaT细胞凋亡的分子机制. 采用Hoechst 33258染色结合琼脂糖凝胶电泳分析细胞凋亡; 用RT-PCR法和细胞免疫荧光染色检测胞内酸性鞘磷脂酶(acid sphingomyelinase, ...
Utilising polyphenylene oxide for high exposure solar UVA dosimetry
polyphenylene oxide solar UVA dosimetry
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2008/11/7
A personal UV dosimeter that can quantitatively assess high exposure solar UVA exposures has been developed. The chemical polyphenylene oxide has been previously reported on its ability to measure hig...
酸性鞘磷脂水解酶和MAPK信号通路在UVA诱导细胞凋亡中的作用
紫外辐射 细胞凋亡 酸性鞘磷脂水解酶 MAPK信号通路
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2007/12/24
摘要 为了探讨酸性鞘磷脂水解酶 (ASM)和MAPK信号通路在UVA诱导的细胞凋亡中的作用 ,用DNA梯形条带 (DNAladder)和荧光显微镜鉴定细胞凋亡 ,Western印迹分析MAPK信号通路的激活情况 .结果显示 :①经UVA照射 ,正常的淋巴母细胞JY出现严重的细胞凋亡 ,而ASM遗传性缺陷的淋巴母细胞MS1 4 1 8出现轻微凋亡 ;给予ASM特异性抑制剂NB6 ,UVA诱导的JY细...