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Do Ligand Binding and Solvent Exclusion Alter the Electrostatic Character within the Oxyanion Hole of an Enzymatic Active Site
Pseudomonas putida Thiocyanates Anions Oxygen Steroid Isomerases Ligands Spectroscopy, Fourier Transform Infrared Kinetics Binding Sites Static Electricity
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2016/5/24
The thousands of enzyme structures solved to date have consistently revealed that biological catalysis occurs within sequestered pockets containing complex interdigitations of polar and hydrophobic gr...
Electric Fields at the Active Site of an Enzyme:Direct Comparison of Experiment with Theory
Humans Nitriles Enzyme Inhibitors Spectrophotometry, Infrared Spectrum Analysis Circular Dichroism Electricity Protein Conformation Protein Structure,Tertiary Protein Folding
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2016/5/24
The electric fields produced in folded proteins influence nearly every aspect of protein function. We present a vibrational spectroscopy technique that measures changes in electric field at a specific...