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微卫星PCR产物变性与非变性PAGE-银染检测方法的比较The Comparision of Denatured and non-denatured PAGE–Silver- Staining Detection of PCR Product of Microsatellites
微卫星 PAGE PCR 银染
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2008/1/8
摘要
用鸡的微卫星引物对6个中国地方鸡种的两个微卫星位点进行PCR扩增。将扩增产物在变性与非变性聚丙烯酰胺凝胶（PAGE）上进行电泳，经银染，表明微卫星产物在二者上的电泳结果有明显的差异。在非变性聚丙烯酰胺凝胶中，表现为有较多的非特异带，而在变性胶中微卫星扩增产物条带清晰，易于鉴定。Abstract：The genomic DNAs from six chicken breeds in Chin...
Molecular Characterisation of Nilagiri Sheep (Ovis aries) of South India Based on Microsatellites
Genetic Characterisation Nilagiri Sheep Microsatellites PIC Inbreeding Estimates
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2016/4/25
Genetic variation in Nilagiri sheep, the only apparel wool breed in South India was studied using 25 FAO recommended ovine-specific microsatellite markers. The number of observed alleles ranged from 3...