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Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
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2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Isolation and Characterization of a New Methanobacterium formicicum KOR-1 from an Anaerobic Digester Using Pig Slurry
Methanogen Anaerobic Digester 16S rRNA mcrA Methanobacterium formicicum
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2016/5/20
A new methanogen was isolated from an anaerobic digester using pig slurry in South Korea. Only one strain, designated KOR-1, was characterized in detail. Cells of KOR-1 were straight or crooked rods, ...
Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities
Brilliant Cresyl Blue Embryonic Development Subcellular Characteristics
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2016/5/19
The in vitro maturation (IVM) efficiency of porcine embryos is still low because of poor oocyte quality. Although brilliant cresyl blue positive (BCB+) oocytes with low glucose-6-phosphate dehydrogena...
Sequencing and Characterization of Divergent Marbling Levels in the Beef Cattle (Longissimus dorsi Muscle) Transcriptome
Marbling Beef Cattle Transcriptome RNA-Sequencing
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2016/5/17
Marbling is an important trait regarding the quality of beef. Analysis of beef cattle transcriptome and its expression profile data are essential to extend the genetic information resources and would ...
Isolation and Characterization of Novel Denitrifying Bacterium Geobacillus sp. SG-01 Strain from Wood Chips Composted with Swine Manure
Geobacillus thermodenitrificans Nitrates Nitrites Swine Manure Wood Chips
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2016/5/12
Nitrate contamination in ground and surface water is an increasingly serious environmental problem and only a few bacterial strains have been identified that have the ability to remove nitrogen pollut...
Molecular Characterization and Expression Analysis of S6K1 in Cashmere Goats (Capra hircus)
Inner Mongolia Cashmere Goat S6K Expression Pattern U0126
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2016/5/12
p70 ribosomal S6 kinase (p70S6K) can integrate nutrient and growth factor signals to promote cell growth and survival. We report our molecular characterization of the complementary DNA (cDNA) that enc...
Molecular Characterization of Selected Local and Exotic Cattle Using RAPD Marker
RAPD-PCR Genetic Distance Polymorphism Cattle Bangladesh
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2016/5/10
In order to develop specific genetic markers and determine the genetic diversity of Bangladeshi native cattle (Pabna, Red Chittagong) and exotic breeds (Sahiwal), randomly amplified polymorphic DNA (R...
Molecular Characterization and Expression of LDHA and LDHB mRNA in Testes of Japanese Quail (Coturnix japonica)
Lactate Dehydrogenase Testes Lactate Isozymes Messenger RNA
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2016/5/6
The LDH isozymes are key catalysts in the glycolytic pathway of energy metabolism. It is well known that the distribution of the LDH isozymes vary in accordance with the metabolic requirements of diff...
Development and Characterization of a Specific Anti-Caveolin-1 Antibody for Caveolin-1 Functional Study in Human, Goat and Mouse
Caveolin-1 Peptide Antibody Breast Cancer Goat Cancer Marker
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2016/4/25
Caveolin-1 of the caveolin family of proteins regulates mammary gland development and has been shown to play a contradictory role in breast tumor progression. A specific anti-Caveolin-1 antibody will ...
Characterization of Indian Riverine Buffaloes by Microsatellite Markers
Characterization Buffalo Genetic Distance Microsatellite
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2016/4/22
Six breeds of riverine buffalo viz. Murrah, Mehsana, Jaffrabadi, Nagpuri, Nili-Ravi and Bhadawari were characterized using FAO-recommended cattle specific microsatellite markers. Among the total of tw...
Molecular Characterization and Expression Patterns of Porcine Eukaryotic Elongation Factor 1 A
EEF1A1 Expression Chromosome Genome Porcine
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2016/4/21
The eukaryotic elongation factor 1 A (EEF1A) participates in protein synthesis by forming the eEF1A쨌GTP쨌tRNA complex to deliver aminoacyl-tRNA to the A site of ribosomes. This study desc...
Characterization of Lactic Bacterial Strains Isolated from Raw Milk
Lactobacillus Probiotics Artificial Gastric Juice rDNA Antibiotic
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2016/4/20
During lactic acid bacteria (LAB) transit through the gastrointestinal tract, ingested microorganisms were exposed to successive stress factors, including low pH in the human stomach and in bile acid....
Identification and Characterization of Hydrogen Peroxide-generating Lactobacillus fermentum CS12-1
Lactic Acid Bacteria Lactobacillus fermentum Hydrogen Peroxide Probiotics
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2016/4/18
Lactic acid bacteria were isolated from silage, which produce high level of hydrogen peroxide in cell culture supernatant. The 16S rDNA sequences of the isolate matched perfectly with that of Lactobac...
Screening and Characterization of Lactate Dehydrogenase-producing Microorganism
Lactobacillus Lactate Dehydrogenase Direct-fed Microbials
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2016/4/18
The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate c...
Protease Inhibitors in Porcine Colostrum: Potency Assessment and Initial Characterization
Porcine Colostrum Protease Inhibitors Growth Factors GI Tract
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2016/4/14
Porcine colostrum and milk were separated into the acid-soluble and casein fractions by acidification followed by centrifuge. The acid-soluble fraction of porcine colostrum was further separated by li...