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Isolation and In vitro Culture of Pig Spermatogonial Stem Cell
Spermatogonial Stem Cell SSC Pluripotency Long Term Culture Pig
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2016/4/29
The present study identified the favorable conditions for isolation, enrichment and in vitro culture of highly purified, undifferentiated pig spermatogonial stem cell (SSC) lines that proliferate for ...
Isolation, Culture and Identification of Porcine Skeletal Muscle Satellite Cells
Pig Skeletal Muscle Satellite Cell Isolation Culture Characterization
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2016/5/18
The objective of this study was to establish the optimum protocol for the isolation and culture of porcine muscle satellite cells. Mononuclear muscle satellite cells are a kind of adult stem cell, whi...
Spatial Heterogeneity of Bacteria: Evidence from Hot Composts by Culture-independent Analysis
Spatial Heterogeneity 16S rRNA Gene Swine Manure Composting)
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2016/5/10
The phylogenetic diversity of the bacteria in hot composting samples collected from three spatial locations was investigated by molecular tools in order to determine the influence of gradient effect o...
Growth of Ovarian Primary Follicles Retrieved from Neonates of Different Ages and Derivation of Mature Oocytes Following In vitro-Culture
Neonatal Mouse Ovary Primary Follicle In vitro Culture Oocyte Maturation
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2016/5/10
This study was conducted to improve the yield of mature oocytes from in vitro-culture of ovarian primary follicles by optimizing follicle retrieval from neonatal mice of different ages. Primary follic...
Effect of Excessive Vitamin A on Alkaline Phosphatase Activity and Concentrations of Calcium-binding Protein and Bone Gla-protein in Culture Medium and CaBP mRNA Expression in Osteoblasts of Broiler Chickens
Vitamin A Broiler Chicken Calcium-binding Protein mRNA Expression Osteoblast Bone Gla-protein
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2016/5/6
This study was conducted to determine the effects of excess vitamin A on alkaline phosphatase (ALP) activity, contents of calcium-binding protein (CaBP), bone gla-protein (BGP) in culture medium and C...
Validation of Methods for Isolation and Culture of Alpaca Melanocytes: A Novel Tool for In vitro Studies of Mechanisms Controlling Coat Color
Alpaca Alpha Melanocyte Stimulating Hormone Coat Color Melanocortin 1 Receptor Melanocyte Pigmentation
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2016/5/4
The objective of the present studies was to develop and validate a system for isolation, purification and extended culture of pigment-producing cells in alpaca skin (melanocytes) responsible for coat ...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
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2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
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2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes
In vitro Fertilization Pig Oocyte Liquid Boar Sperm
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2016/4/18
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
The Investigation of Cell Culture Conditions to Maintain Chicken Embryonic Stem Cells as Totipotent Cells
Embryonic Stem Cells Feeder Layer Cells Chicken ES Cells Chimeras
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2016/4/13
The ES cell can provide a useful system for studying differentiation and development in vitro and a powerful tool for producing transgenic animalds. To investigate the culture condition of chicken emb...
Biochemical Compositions of Follicular Fluid and the Effects of Culture Conditions on the In Vitro Development of Pig Oocytes
Protein Lipid Cholesterol Sex Hormones IVMFC
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2016/4/12
The aims of this study were, firstly, to analyze the biochemical compositions of serum and follicular fluid (FF) from prepubertal gilts after PMSG (1,000 IU) treatment. The concentrations of total pro...
Effect of Mitogens, Supplement of -mercaptoethanol and Fetal Bovine Serum Supplementation in Whole Blood Culture Medium for Bovine Chromosome Analysis
Bovine Chromosome Analysis #NAME? Mitogens Metaphase Plates
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2016/4/8
We investigated the effects of the mitogen supplements of 3 types, pokeweed mitogen (PWM), phytohemagglutinin (PHA) and concanavalin A (ConA), to a whole blood culture system on the number of metaphas...
Secretory Proteins from Goat Oocytes Matured in Culture
Granulosa Cell Monolayer Polyvinyl Alcohol Oocyte Secretory Proteins IVM
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2016/4/8
In this experiment, oocytes were collected from goat ovaries available in slaughterhouse by follicle puncture method. Morphologically culturable type of oocytes which having compact, multilayered cumu...