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Expression and processing of xanthin dehydrogenase/oxidase cDNA from African cape buffalo
Biological sciences Xanthin dehydrogenase Syncerus caffer Trypanosome Cape buffalo
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2015/1/28
Our lab studies oxidative regulation in defense against extracellular pathogens, especially African Trypanosomes. ^ Trypanosomes are protozoan hemoflagellates. The organisms are transmitted by tsetse ...
人α-型肿瘤坏死因子cDNA在大肠杆菌中的高效表达
肿瘤坏死因子 基因表达 P_L启动子
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2007/12/24
摘要 人α-型肿瘤坏死因子(Tumor Necrosis Factor,TNF-α)可以在体内或体外特异地杀伤多种肿瘤细胞,并同时具有抗病毒感染及免疫调节活性,是一种非常有前途的抗肿瘤及抗病毒生物制剂。最近,国外用基因工程技术将TNF-α基因克隆并在大肠杆菌及酵母细胞中表达成功。目前,人们正致力于提高其在大肠杆菌中表达产量的研究。据此,我们构建了P_L启动子控制的TNF-α cDNA表达载体,使其...
利用真菌纤维素结合域(CBD)保守性序列进行草菇木聚糖酶cDNA的克隆
Volvariella volvacea cellulose-binding domain(CBD) xylanase cDNA
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2007/12/21
Cellulose binding domains (CBDs) are present in the majority of fungal cellulases and hemicellulases. Based on the conserved region of CBDs, degenerate primers were designed and used to amplify the 5′...