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Characterization of the Mitochondrial Proteome in Pyruvate Dehydrogenase Kinase 4 Wild-Type and Knockout Mice
Starvation Mitochondria Proteomics PDK4
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2015/5/25
The goal of this study was to determine the effect of a PDK4 (pyruvate dehydrogenase kinase isoenzyme 4) knock-out on mitochondrial protein expression. A 2-D gel based mass spectrometry approach was u...
山羊β乳球蛋白基因的克隆及其在转基因小鼠乳汁中的高效表达Goat β-lactoglobulin Gene Cloning and High Expression in the Mammary Gland of Transgenic Mice
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2007/12/31
摘要通过长距离PCR从山羊基因组DNA分两段扩增山羊β乳球蛋白(β-lactoglobulin,BLG)基因,扩增出的两个片段分别克隆到T载体上,利用BLG基因序列自身存在的NarI单酶切位点进行拼接,获得了全长为7.2kb的山羊BLG基因克隆,并构建了它的真核表达载体,经酶切鉴定和序列分析证实了克隆的正确性。用线性化的BLG基因显微注射小鼠受精卵以建立转基因鼠,经PCR和Southern印迹分析...
Systemic infusion of Flk1 mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice.
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2007/7/28
专著信息
书名
Systemic infusion of Flk1 mesenchymal stem cells ameliorate carbon tetrachloride-induced liver fibrosis in mice.
语种
英文
撰写或编译
撰写
作者
Fang B,Shi M,Zhao RCH
第一作者单位
出版社
Transplantation. 15;78(1):8...
![](http://www.firstlight.cn/upload/imgfile/20070924d/113181a35606.gif)
Mice and men make livers differently(图)
gene liver cells
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2007/5/22