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旨在构建干扰胰岛素样生长因子（insulin-like growth factor-II，IGF-II）的重组质粒载体，研究IGF-II对牦牛睾丸支持细胞的影响。本研究设计并合成针对牦牛IGF-II的shRNA寡核苷酸，并将其克隆至pLKO.1质粒载体上，转染牦牛睾丸支持细胞后经嘌呤霉素筛选获得稳定的细胞株，并采用实时荧光定量PCR（qRT-PCR）和蛋白免疫印迹法检测IGF-II基因和蛋白的表达...
Short communication. An association between the G/A single nucleotide polymorphism within intron II of VIP gene and milk performance traits in dairy cattle
vasoactive intestinal peptide lactation milk utility traits
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2014/10/28
Single nucleotide polymorphisms (SNPs), which are present in the encoding part of the genes responsible for important breeding functions, exert an influence on the cattle’s phenotype since their funct...
Statistical Genetic Studies on Cattle Breeding for Dairy Productivity in Bangladesh: II. Estimation of Reciprocal and Heterosis Effects and Optimum Crossbreeding System between the Local Breeds and Exotic Breeds for Milk Performance
Bangladeshi Cattle Reciprocal Effects Heterosis Optimum Crossbreeding System
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2016/4/11
Reciprocal effects among local breeds and additive and heterosis effects were estimated for total milk production per lactation (TLP), lactation length (LL) and daily milk yield (DMY). Procedures for ...