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[ 目的] 运用分子生物学技术克隆猪GPX2 基因。[ 方法] 以猪十二指肠总RNA 为模板, 根据人、大鼠、小鼠、牛、狗GPX2 基因同源
序列分析, 设计兼并引物对, 采用RT- PCR 技术扩增获得了1 段330bp 的猪GPX2 基因序列。根据获得的已知序列分别设计引物, 采用
3′- RACE 和5′- RACE 技术手段分离、克隆猪GPX2 基因。并对所得基因进行基因序列分析。[ ...
Study of the leucocytic formula of milk in the ewes of race ouled-djellal in the east of Algeria
leucocytic formula milk east of Algeria
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2009/3/4
The study was carried out in a private ovine exploitation of Ben M’ Hidi (wilaya of El-Tarf), located at northeast of Algeria during the period going from June 2000 to May 2002. It related to 13 n...