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Comparison of Conventional Cytogenetic and Fluorescence In Situ Hybridization Results of Prenatal Aneuploidy Screening
Aneuploidy prenatal diagnostic tests FISH conventional cytogenetics
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2009/6/17
Background and Aim: Prenatal diagnosis of chromosome abnormalities is usually performed on amniotic fluid samples. Aneuploidy screening by fluorescence in situ hybridization (FISH), although not an al...
用间期荧光原位杂交检测卵巢癌细胞中X染色体数目的异常The Application of Interphase Fluorescence in situ Hybridization to the Diagnosis of X Chromosomal Count Abnormality in Ovarian Carcinoma Cell
荧光原位杂交 卵巢癌 X染色体数目异常 DNA特异性探针
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2008/1/10
摘要为了探讨用荧光原位杂交技术（fluorescence in situ hybridization, FISH）检测卵巢癌细胞中性染色体拷贝数目异常的实验方法及其应用价值，收集18例新鲜卵巢癌组织标本，以Biotin标记的X染色体α-卫星DNA（pBamX7）探针与经处理的标本进行卵巢癌细胞核的原位杂交，分别用Avidin-FITC和Anti-avidin进行信号的检测与放大，PI复染。于Oly...