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Current evaluation of male fertility, routinely estimated by sperm count, motility, and morphology, provides only crude information about the fertility state of individuals. Both flow and image cyto...
Tumor necrosis factor (TNF alpha) is present in elevated levels in peritoneal fluid from infertile women with endometriosis. The effect of TNF alpha on human sperm motility in vitro was evaluated ...
A synchronous acrosome reaction system was established for human spermatozoa. Seminal plasma is removed from the spermatozoa by centrifugation and the washed spermatozoa are capacitated in a modif...
Multivariate discriminant analysis was used to evaluate the usefulness of computer image analysis of sperm movement characteristics and differential patterns of sperm tail swelling after hypoosmot...
Previous results, simultaneously confirmed by others, suggest that a relationship exists between sperm acrosin levels and fertility in man. The assessment of sperm acrosin may therefore be a useful ...
The conventional sperm characteristics of density (millions per milliliter) and motility, scored in a semi-subjective way, were correlated with results of an on-going in vitro fertilization and em...
Semen from 63 individuals participating in an in vitro fertilization program was processed using a modified rise technique. Overall normal morphology was significantly improved in the rise (79.2%) as ...
Human ejaculates (n = 83) were analyzed for standard sperm parameters (concentration, motility, and morphology), as well as for the ability of the spermatozoa to react (swell) in a hypoosmotic mediu...
Time exposure photomicrography and interspecific in vitro fertilization techniques have been used to compare the responses to the divalent cation ionophore A23187 of spermatozoa from normal fertile an...
Penetration of spermatozoa in bovine cervical mucus (BCM) from two groups of men was investigated and correlated with the different parameters of spermograms from these same subjects. Sperm penetr...
Sperm penetration into the zona of 46 human eggs was examined by electron microscopy. Preovulatory oocytes were aspirated at laparoscopy and inseminated by methods which produced normal pregnancie...
Fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC) were evaluated for use as contrasting fluorescent labels on living human spermatozoa. Unlabeled (control), FITC...
Acrosin is a sperm serine proteinase whose activity appears to be essential for fertilization in mammals, although this has not been shown in man due to experimental limitations. The present stu...
Recently, there is increasing evidence suggesting that oxidative sperm DNA damage is closely associated with impaired sperm function and male infertility. 8-Hydroxydeoxyguanosine (8-OHdG) is consi...
Prosaposin is a well-characterized, approximately 68-kDa protein found in many tissues and as a normal component of human semen. A fragment of prosaposin apparently is involved in primary sperm-eg...

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