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Effects of IGF-I and EGF Supplemented to PZM3 Culture Medium on the Development of Porcine Embryos In vitro
Embryo Development Porcine Embryo Growth Factor PZM3
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2016/4/29
This study investigated the effects of IGF-I and EGF on the development of blastocysts or hatched blastocysts during the in vitro culture of embryos from immature porcine oocytes. After the in vitro m...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
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2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Emil Behring's Medical Culture:From Disinfection to Serotherapy
Emil Behring's Medical Culture Disinfection to Serotherapy
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2009/1/4
The introduction of the first serum therapies for the treatment of diphtheria and tetanus in the mid-1890s constitutes an important development in the history of medicine. The technique—pioneered by E...
Effects of Yeast Culture Supplementation to Gestation and Lactation Diets on Growth of Nursing Piglets
Yeast Culture Sow Litter Weight Gain
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2016/4/28
A total of 335 sows at a commercial operation (Hitch Pork Producers Inc, Guymon, OK) was used to determine dietary effects of yeast culture supplementation (XPCTM, Diamond V Mills) on litter performan...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
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2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
In vitro Culture Conditions for the Mouse Preantral Follicles Isolated by Enzyme Treatment
Chromatin Pattern Media Mouse Preantral Follicle Protein
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2016/4/27
In order to investigate the factors affecting the culture of mouse preantral follicles in vitro, we examined the effect of culture media, protein supplements, and culture period on their growth. The o...
一种早期胚胎体外培养新方法的建立Establishment of a New Method for Early Embryo Culture in vitro
体外培养 套皿法 早期胚胎
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2008/1/8
摘要
建立一种不依赖于液体石蜡的早期胚胎体外培养方法——套皿法,并比较了4种不同处理方法体外培养胚胎的效果。结果显示,采用套皿法进行胚胎培养,盖液体石蜡和不盖液体石蜡皿中的胚胎在各阶段的发育率差异不显著。与套皿法相比,用单皿微滴覆盖法(Brinster法)培养的胚胎在各阶段的发育率显著降低。不覆盖液体石蜡单皿中的胚胎则阻断于二细胞阶段。本实验设计的套皿法是一种有效的早期胚胎体外培养方法,为早期...
Developmental Competence of Intrafollicular Oocytes Derived from Preantral Follicle Culture with Different Protocols after Parthenogenetic Activation
Mouse Secondary Follicle Intrafollicular Oocyte Maturation Parthenogenesis Blastocyst Formation
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2016/4/26
This study was conducted to improve efficiency of a follicle culture system without reducing developmental competence of intrafollicular oocytes. Preantral follicles (100 to 125 m in diameter)...
Retroviral Gene Expression in Spermatogonial Stem Cells during Long-term Culture
Retrovirus Gene Transfer Electroporation Stem Cell Medium
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2016/4/26
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be re...
The relative significance of viral lysis and microzooplankton grazing as pathways of dimethylsulfoniopropionate (DMSP) cleavage: An Emiliania huxleyi culture study
viral lysis microzooplankton grazing pathways of dimethylsulfoniopropionate Emiliania huxleyi culture
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2014/4/22
Dimethylsulfoniopropionate (DMSP) cleavage was investigated during culture studies of grazing by the microzooplankter Oxyrrhis marina and viral lysis by Emiliania huxleyi virus 86 (EhV-86) on two axen...
Uptake of Spartina-derived humic nitrogen by estuarine phytoplankton in nonaxenic and axenic culture
Spartina-derived humic nitrogen estuarine phytoplankton nonaxenic and axenic culture
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2014/5/5
Humic substances are a collection of colored organic acids characterized by high molecular weight and low nitrogen (N) content that are thought to be biologically recalcitrant. We examined a suite of ...
Effect of Protein Sources on Rumen Microbial Protein Synthesis Using Rumen Simulated Continuous Culture System
RSCC Urea Casein Soy Protein Microbial Protein Synthesis N Flow
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2016/4/19
A rumen simulated continuous culture (RSCC) system was used to study the influence of supplementation of the three different types of protein sources such as urea, casein and soy protein on rumen micr...
Diurnal cycling of glutathione in marine phytoplankton: Field and culture studies
glutathione marine phytoplankton Field culture
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2014/5/19
Glutathione is an abundant intracellular thiol that is involved in the detoxification of reactive oxygen species generated in the presence of light. We used short-term continuous cultures of Emiliania...
Liquid Boar Sperm Quality during Storage and In vitro Fertilization and Culture of Pig Oocytes
In vitro Fertilization Pig Oocyte Liquid Boar Sperm
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2016/4/18
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
Influence of Supplemental Enzymes, Yeast Culture and Effective Micro-organism Culture on Gut Micro-flora and Nutrient Digestion at Different Parts of the Rabbit Digestive Tract
Rabbit Digestion Enzymes Probiotics Gut-microflora
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2016/4/15
An experiment of 10 weeks duration was carried out to study the influence of supplemental effective microorganism (EM) culture, yeast culture and enzymes on nutrient digestibility and gut microflora i...