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This study investigated the effects of IGF-I and EGF on the development of blastocysts or hatched blastocysts during the in vitro culture of embryos from immature porcine oocytes. After the in vitro m...
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
The introduction of the first serum therapies for the treatment of diphtheria and tetanus in the mid-1890s constitutes an important development in the history of medicine. The technique—pioneered by E...
A total of 335 sows at a commercial operation (Hitch Pork Producers Inc, Guymon, OK) was used to determine dietary effects of yeast culture supplementation (XPCTM, Diamond V Mills) on litter performan...
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
In order to investigate the factors affecting the culture of mouse preantral follicles in vitro, we examined the effect of culture media, protein supplements, and culture period on their growth. The o...
摘要 建立一种不依赖于液体石蜡的早期胚胎体外培养方法——套皿法,并比较了4种不同处理方法体外培养胚胎的效果。结果显示,采用套皿法进行胚胎培养,盖液体石蜡和不盖液体石蜡皿中的胚胎在各阶段的发育率差异不显著。与套皿法相比,用单皿微滴覆盖法(Brinster法)培养的胚胎在各阶段的发育率显著降低。不覆盖液体石蜡单皿中的胚胎则阻断于二细胞阶段。本实验设计的套皿法是一种有效的早期胚胎体外培养方法,为早期...
This study was conducted to improve efficiency of a follicle culture system without reducing developmental competence of intrafollicular oocytes. Preantral follicles (100 to 125 m in diameter)...
The spermatogonial stem cell (SSCs) is unique in that it is the only cell in the adult male that can contribute genes to a subsequent generation. Permanent modification of the germ cell line may be re...
Dimethylsulfoniopropionate (DMSP) cleavage was investigated during culture studies of grazing by the microzooplankter Oxyrrhis marina and viral lysis by Emiliania huxleyi virus 86 (EhV-86) on two axen...
Humic substances are a collection of colored organic acids characterized by high molecular weight and low nitrogen (N) content that are thought to be biologically recalcitrant. We examined a suite of ...
A rumen simulated continuous culture (RSCC) system was used to study the influence of supplementation of the three different types of protein sources such as urea, casein and soy protein on rumen micr...
Glutathione is an abundant intracellular thiol that is involved in the detoxification of reactive oxygen species generated in the presence of light. We used short-term continuous cultures of Emiliania...
The percentages of sperm motility and normal acrosome on the liquid boar semen diluted and preserved at 4 C with lactose hydrate, egg yolk and N-acetyl-D-glucosamine (LEN) diluent were significant dif...
An experiment of 10 weeks duration was carried out to study the influence of supplemental effective microorganism (EM) culture, yeast culture and enzymes on nutrient digestibility and gut microflora i...

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