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顾宏周课题组《STAR Protocols》报道制备长单链DNA的生物法新策略（图）
单链DNA序列 顾宏周课题组 蛋白酶
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2023/4/17
当前，单链DNA序列的体外获取严重依赖于固相化学合成，其技术上已非常成熟，但依然具有明显的局限性。比如，化学合成DNA的产率随着序列长度的增加而显著降低，当超过200个碱基时，化学合成得到的DNA不仅产率/量低下，而且成本较高。换言之，化学合成长单链DNA的性价比很低，该缺点严重限制了很多基于长单链DNA序列的下游生物技术的应用，如长单链DNA模板介导的基因敲入、基于长单链DNA锁式探针的长程测序...
猪StAR基因启动子区克隆及其转录活性研究
StAR基因 启动子 荧光素酶活性 报告基因
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2018/7/26
旨在通过分析猪StAR基因启动子活性区域，探究猪StAR基因的转录调控机制，从育种学角度为提高猪繁殖力提供新思路。本研究根据Ensembl数据库已公布的猪StAR基因的5'侧翼区序列，利用在线预测软件对该基因启动子区序列信息进行分析，以大白猪基因组DNA为模板，利用特异性引物，进行PCR扩增、测序，进而构建启动子区不同缺失片段的pGL3-StAR双荧光素酶表达载体，转染293T细胞并进行活性检测。...
THERMAL EFFECTS ON CAMERA FOCAL LENGTH IN MESSENGER STAR CALIBRATION AND ORBITAL IMAGING
Mercury, MESSENGER, MDIS Camera Digital Terrain Models
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2018/5/11
We analyse images taken by the MErcury Surface, Space ENviorment, GEochemistry, and Ranging (MESSENGER) spacecraft for the camera’s thermal response in the harsh thermal environment near Mercury. Spec...
THE HODGE STAR OPERATOR ON SCHUBERT FORMS
SCHUBERT FORMS THE HODGE STAR OPERATOR
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2015/12/17
Let X = G=P be a homogeneous space of a complex semisimple
Lie group G equipped with a hermitian metric. We study the action of the
Hodge star operator on the space of harmonic di erential forms on ...
Star formation in bright-rimmed clouds. I. Millimeter and submillimeter molecular line surveys
pre-main sequence clouds
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2014/12/26
We present the results of the first detailed millimeter and submillimeter molecular line survey of bright-rimmed clouds, observed at FCRAO in the CO (J = 1 → 0), C18O (J = 1 → 0), HCO+ (J = 1 → 0), H1...
Star formation in bright-rimmed clouds. I. Millimeter and submillimeter molecular line surveys
pre-main sequence clouds
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2014/12/26
We present the results of the first detailed millimeter and submillimeter molecular line survey of bright-rimmed clouds, observed at FCRAO in the CO (J = 1 → 0), C18O (J = 1 → 0), HCO+ (J = 1 → 0), H1...
Radio and Far-Infrared Emission as Tracers of Star Formation and Active Galactic Nuclei in Nearby Cluster Galaxies
AWM7 A426
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2014/12/24
We have studied the radio and far-infrared (FIR) emission from 114 galaxies in the seven nearest clusters (<100 Mpc) with prominent X-ray emission to investigate the impact of the cluster environment ...
The interplay between star formation and the nuclear environment of our Galaxy: deep X-ray observations of the Galactic centre Arches and Quintuplet clusters
n star formation the nuclear environment
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2014/12/24
The Galactic centre (GC) provides a unique laboratory for a detailed examination of the interplay between massive star formation and the nuclear environment of our Galaxy. Here, we present a 100-ks Ch...
Entrainment Mechanisms for Outflows in the L1551 Star-Forming Region
L1551 IRS5 formation
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2014/12/24
We present high sensitivity 12CO and 13CO J=1!0 molecular line maps covering the full extent of the parsec scale L1551 molecular outflow, including the redshifted east-west (EW) flow. We also present ...
Star formation and feedback in smoothed particle hydrodynamic simulations - I. Isolated galaxies
star formation supernova feedback
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2014/12/24
We present an analysis of star formation and feedback recipes appropriate for galactic smoothed particle hydrodynamics simulations. Using an isolated Milky Way-like galaxy, we constrain these recipes ...
The morphological diversities among star-forming galaxies at high redshifts in the great observatories origins deep survey
high-redshift fundamental parameters
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2014/12/24
We used the deep, multiwavelength images obtained by the Great Observatories Origins Deep Survey (GOODS) to identify ~4700 Lyman break galaxies (LBGs) at z > 2.5, and 292 starburst galaxies at z ~ 1.2...
The incidence of highly obscured star-forming regions in SINGS galaxies
photometry ISM
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2014/12/23
Using the new capabilities of Spitzer and extensive multiwavelength data from SINGS, it is now possible to study the infrared properties of star formation in nearby galaxies down to scales equivalent ...
Star formation in NGC 5194 (M51a). II. The spatially resolved star formation law
galaxies formation
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2014/12/23
We have studied the relationship between the star formation rate (SFR), surface density, and gas surface density in the spiral galaxy M51a (NGC 5194), using multiwavelength data obtained as part of th...
The calibration of mid-infrared star formation rate indicators
starburst interactions
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2014/12/23
With the goal of investigating the degree to which the MIR emission traces the SFR, we analyze Spitzer 8 and 24 μm data of star-forming regions in a sample of 33 nearby galaxies with available HST NIC...
Measuring star formation in local and distant galaxies
local distant galaxies
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2014/12/23
I review measurements of star formation in nearby galaxies in the UV-to-FIR wavelength range, and discuss their impact on SFR determinations in intermediate and high redshift galaxy populations. Exist...